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Template:Team:KULeuven/9 September 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
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# motherplate for {{kulpart|BBa_J23101}} was made | # motherplate for {{kulpart|BBa_J23101}} was made | ||
# liquid cultures for checking the ligX were made. | # liquid cultures for checking the ligX were made. | ||
| - | # LC with {{kulpart|BBa_J23101}} were miniprepped, nanodropped, restrictie digested and put on gel. the signals were good. the vector, pSB1A2, was cut from the gel and gel extracted. this was ligated with {{kulpart|BBa_K238013}}. | + | # LC with {{kulpart|BBa_J23101}} were miniprepped, nanodropped, restrictie digested and put on gel. the signals were good. the vector, pSB1A2, was cut from the gel and gel extracted (10ng/µl). this was ligated with {{kulpart|BBa_K238013}}. |
nanodrop: | nanodrop: | ||
{| border ="1" align="center" | {| border ="1" align="center" | ||
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|- align="center" | |- align="center" | ||
| {{kulpart|BBa_J23101}}(4) || 141 || 1,95 || | | {{kulpart|BBa_J23101}}(4) || 141 || 1,95 || | ||
| - | |} | + | |} |
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Revision as of 16:26, 10 September 2009
- FACS-ing the liquid cultures that were put under blue light on 8/09 gave no significant results.
- motherplate for was made
- liquid cultures for checking the ligX were made.
- LC with were miniprepped, nanodropped, restrictie digested and put on gel. the signals were good. the vector, pSB1A2, was cut from the gel and gel extracted (10ng/µl). this was ligated with .
nanodrop:
| Part | concentration (ng/μl) | 260/280 λ | |
|---|---|---|---|
| (1) | 141,8 | 1,94 | |
| (2) | 139,5 | 1,99 | |
| (3) | 177,5 | 1,95 | |
| (4) | 141 | 1,95 |
