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Template:Team:KULeuven/10 September 2009/VanillinProduction
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(New page: * Gel electrophoresis of TER restriction ** The vector for the TER is Psb1AK3 and has 3100bp, with insert is ±3200 bp ** Gel extraction {| border ="1" align="center" !| part || concentra...)
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(New page: * Gel electrophoresis of TER restriction ** The vector for the TER is Psb1AK3 and has 3100bp, with insert is ±3200 bp ** Gel extraction {| border ="1" align="center" !| part || concentra...)
Newer edit →
Revision as of 16:39, 10 September 2009
- Gel electrophoresis of TER restriction
- The vector for the TER is Psb1AK3 and has 3100bp, with insert is ±3200 bp
- Gel extraction
| part | concentration | 260/280 | 260/230 |
|---|---|---|---|
| TER | 14,2 | 1,97 |
- Created a mother plate from EF1
- Ligated EF restriction + TER restriction
- Transferred the SAMS 1 and SAMS 2 colonies to liquid medium
