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Team:Imperial College London/Drylab
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Large scale automation of production of protein of interest relies on being able to control when the manufacturing process begins. To do this, the initial design consisted of genetic constructs ([timers]). After implementing the simulations and obtaining design constraints, we realized that they were too complicated for the system. Instead, we chose to focus on autoinduction media, where we relied on primary and secondary consumption of sugars to control both the production of protein of interest and the encapsulation process. | Large scale automation of production of protein of interest relies on being able to control when the manufacturing process begins. To do this, the initial design consisted of genetic constructs ([timers]). After implementing the simulations and obtaining design constraints, we realized that they were too complicated for the system. Instead, we chose to focus on autoinduction media, where we relied on primary and secondary consumption of sugars to control both the production of protein of interest and the encapsulation process. | ||
| - | ===Module 1 | + | ===Module 1: Protein production models=== |
Production of Protein of interest via LacI-IPTG induction | Production of Protein of interest via LacI-IPTG induction | ||
*[https://2009.igem.org/Team:Imperial_College_London/M1/Modelling Overview]<br> | *[https://2009.igem.org/Team:Imperial_College_London/M1/Modelling Overview]<br> | ||
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*[https://2009.igem.org/Team:Imperial_College_London/Drylab/M1/Enzyme_kinetics/Analysis The Model]<br> | *[https://2009.igem.org/Team:Imperial_College_London/Drylab/M1/Enzyme_kinetics/Analysis The Model]<br> | ||
*[https://2009.igem.org/Team:Imperial_College_London/Drylab/M1/Enzyme_kinetics/Simulations Simulations]<br> | *[https://2009.igem.org/Team:Imperial_College_London/Drylab/M1/Enzyme_kinetics/Simulations Simulations]<br> | ||
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===Encapsulation=== | ===Encapsulation=== | ||
Revision as of 14:14, 12 September 2009
Contents |
Drylab Hub
The goal of the Drylab has been to support the Wetlab by answering questions of interest. The conclusions from the models will provide the team with functional understanding of the system, and provide design constraints and considerations that should be taken into account in the genetic constructs.
The Models
In the Drylab, we have implemented several functional models, explaining the behaviour of different parts of the system.This section will provide a summary of the models, with the conclusions drawn from them.
Autoinduction
Large scale automation of production of protein of interest relies on being able to control when the manufacturing process begins. To do this, the initial design consisted of genetic constructs ([timers]). After implementing the simulations and obtaining design constraints, we realized that they were too complicated for the system. Instead, we chose to focus on autoinduction media, where we relied on primary and secondary consumption of sugars to control both the production of protein of interest and the encapsulation process.
Module 1: Protein production models
Production of Protein of interest via LacI-IPTG induction
Enzyme kinetics
Encapsulation
Genomic deletion induced by thermoinduction
