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Team:Chiba/Notebook/Calendar/2 September 2009
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| - | * | + | *prior culture(exploratory experiment) |
| - | + | medium:liquid culture medium antibiotic:Amp | |
| - | + | We took this medium to centrifuging tube 50 mL and added antibiotic(Cm) 50 μL. | |
| - | + | Then we transferred the medium to test tube 2 mL and added glycerol stock(BW25113, ΔTdk,pCIA3-LuxR,pLux-GFP) and then started shake culture at 37 degrees Celsius. | |
| - | + | (21:40-) | |
[[Image:20090903-1.jpg|200px]] [[Image:Chiba_Labwork_02Sep09.jpg|200px]] | [[Image:20090903-1.jpg|200px]] [[Image:Chiba_Labwork_02Sep09.jpg|200px]] | ||
Revision as of 06:26, 14 September 2009
(Lab Work Start! |>3_September_2009)
- prior culture(exploratory experiment)
medium:liquid culture medium antibiotic:Amp
We took this medium to centrifuging tube 50 mL and added antibiotic(Cm) 50 μL.
Then we transferred the medium to test tube 2 mL and added glycerol stock(BW25113, ΔTdk,pCIA3-LuxR,pLux-GFP) and then started shake culture at 37 degrees Celsius. (21:40-)
