Team:Chiba/Notebook/Calendar/2 September 2009

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Revision as of 06:27, 14 September 2009

(Lab Work Start! |>3_September_2009)

prior culture(exploratory experiment)

medium:liquid culture medium antibiotic:Amp

We took this medium to centrifuging tube 50 mL and added antibiotic(Cm) 50 μL.

Then we transferred the medium to test tube 2 mL and added glycerol stock(BW25113, ΔTdk,pCIA3-LuxR,pLux-GFP) and then started shake culture at 37 degrees Celsius. (21:40-)

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	We took this medium to centrifuging tube 50 mL and added antibiotic(Cm) 50 μL.		We took this medium to centrifuging tube 50 mL and added antibiotic(Cm) 50 μL.