Template:Team:KULeuven/12 August 2009/VanillinProduction

From 2009.igem.org

(Difference between revisions)
 
Line 1: Line 1:
-
* Miniprep on Sam5, Sam8 and Fcs
+
* Miniprep on ''sam5, sam8'' and ''fcs''
-
** There was no growth on Ech, thus new cultures in liquid medium
+
** There was no growth on ''ech'', thus new cultures in liquid medium
-
* Restriction digest on Sam5, Sam8 and Fcs
+
* Restriction digest on ''sam5, sam8'' and ''fcs''
-
* Gel electrophoresis on Sam5, Sam8 and Fcs
+
* Gel electrophoresis on ''sam5, sam8'' and ''fcs''
-
* Gel extraction on Sam5 and Fcs
+
* Gel extraction on ''sam5'' and ''fcs''
Nanodrop results:
Nanodrop results:
{| border ="1" align="center"
{| border ="1" align="center"
!| Part || concentration (ng/μl) || 260/280 λ || 260/230 λ
!| Part || concentration (ng/μl) || 260/280 λ || 260/230 λ
|- align="center"
|- align="center"
-
| Fcs ||4,2 || 6,08 ||  
+
| ''fcs'' ||4,2 || 6,08 ||  
|- align="center"
|- align="center"
-
| Sam5 ||7,3|| 2,76 ||   
+
| ''sam5'' ||7,3|| 2,76 ||   
|-
|-
|}
|}
Notes:<br>
Notes:<br>
-
* Sam8 showed a very low concentration on the gel electrophoresis. Thus, it is better to redo this test.
+
* ''sam8'' showed a very low concentration on the gel electrophoresis. Thus, it is better to redo this test.
-
* 260/280 for Fcs was very high and the concentration was not high enough. Therefore, we will redo the extraction and use multiple tubes on the same filter to increase the concentration
+
* 260/280 for ''fcs'' was very high and the concentration was not high enough. Therefore, we will redo the extraction and use multiple tubes on the same filter to increase the concentration

Latest revision as of 09:58, 14 September 2009

  • Miniprep on sam5, sam8 and fcs
    • There was no growth on ech, thus new cultures in liquid medium
  • Restriction digest on sam5, sam8 and fcs
  • Gel electrophoresis on sam5, sam8 and fcs
  • Gel extraction on sam5 and fcs

Nanodrop results:

Part concentration (ng/μl) 260/280 λ 260/230 λ
fcs 4,2 6,08
sam5 7,3 2,76

Notes:

  • sam8 showed a very low concentration on the gel electrophoresis. Thus, it is better to redo this test.
  • 260/280 for fcs was very high and the concentration was not high enough. Therefore, we will redo the extraction and use multiple tubes on the same filter to increase the concentration
Retrieved from "http://2009.igem.org/Template:Team:KULeuven/12_August_2009/VanillinProduction"