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Uppsala-Sweden/15 September 2009
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(New page: {{Uppsala-Sweden Template}} ==Picking colonies and colony PCR== The colonies from the plated transformations sice yesterday was picked this morning, together with the antisense RNA's tha...) |
Karl.brune (Talk | contribs) (→Picking colonies and colony PCR) |
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These colonies were run through a Taq PCR. | These colonies were run through a Taq PCR. | ||
--[[User:Flormane|Flormane]] 12:54, 15 September 2009 (UTC) | --[[User:Flormane|Flormane]] 12:54, 15 September 2009 (UTC) | ||
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| + | ==Gel Colony PCR of GFP and Lux System Parts== | ||
| + | |||
| + | Ai: A340620 + i13504 (2,1kb) | ||
| + | AJ: A340620 + J37034 (2,8kb) | ||
| + | ACO: A340620 + C0261 (1,9kb) | ||
| + | AB: A340620 + B0034 (1,2kb) | ||
| + | Pi: pPetE + i13504 (1,6kb) | ||
| + | |||
| + | [[Image:20090915_GFP_LUX_ColonyPCR-labelled.jpg]] | ||
Revision as of 17:17, 15 September 2009
Picking colonies and colony PCR
The colonies from the plated transformations sice yesterday was picked this morning, together with the antisense RNA's that didn't seemed to have worked according to the evaluation gel from yesterday.
pPetE-E1A1-T, pPetE-E1B1-T, pPetE-E1B3-T, pPetE-E21-T, kivd, RBS-pdc-RBS-adh2z, RBS-kivd, kivd-RBS-YADH2-T, pPetE-RBS and RBS-PirA-RBS-PirB-T.
These colonies were run through a Taq PCR. --Flormane 12:54, 15 September 2009 (UTC)
Gel Colony PCR of GFP and Lux System Parts
Ai: A340620 + i13504 (2,1kb) AJ: A340620 + J37034 (2,8kb) ACO: A340620 + C0261 (1,9kb) AB: A340620 + B0034 (1,2kb) Pi: pPetE + i13504 (1,6kb)
