Team:Imperial College London/Drylab/Enzyme

Kinetics of protein drug

Model for drug protein enzyme kinetics

The protein drug of interest is an enzyme. It will bind to specific substrates and increase the rate of their conversion into products. Therefore, by monitoring either the substrate concentration or the product concentration, we can indirectly see the activity of the enzyme. This is quantified by measuring by enzyme activity in the cellulase assay and PAH assay. Enzyme activity is the rate of substrate utilisation /product formation per unit time.

Our Goals

This model aims to better understand the enzymatic action of the drug by:

• Characterizing its enzymatic activity
• Subsequently,by modeling the relationship between the quantity of protein being produced and the enzyme activity using a simple Michaelis-Menten enzyme kinetics model will enable us to take into account factors that could impair enzymatic activity

This model will allow an understanding of the activity of the enzymes and allow the WETLAB to have an idea of the magnitude of the enzymatic activity. More importantly, after the results from enzyme assays are obtained, the model should provide a means of relating the activity output data to the concentration of the enzyme.

More information on the model can be found in the Drylab hub

Michaelis-Menten enzyme kinetics of protein of interest.

• Overview
  
• The Model
  
• Simulations