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Team:Imperial College London/Drylab/Enzyme
From 2009.igem.org
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(→Model for drug protein enzyme kinetics) |
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<font face='Calibri' size='5'><b>Kinetics of protein drug</b></font> | <font face='Calibri' size='5'><b>Kinetics of protein drug</b></font> | ||
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The protein drug of interest is an enzyme. It will bind to specific substrates and increase the rate of their conversion into products. Therefore, by monitoring either the substrate concentration or the product concentration, we can indirectly see the activity of the enzyme. This is quantified by measuring by enzyme activity in the [https://2009.igem.org/Team:Imperial_College_London/Wetlab/Protocols/cellulase cellulase assay] and [https://2009.igem.org/Team:Imperial_College_London/Wetlab/Protocols/PAH PAH assay]. Enzyme activity is the rate of substrate utilisation /product formation per unit time. | The protein drug of interest is an enzyme. It will bind to specific substrates and increase the rate of their conversion into products. Therefore, by monitoring either the substrate concentration or the product concentration, we can indirectly see the activity of the enzyme. This is quantified by measuring by enzyme activity in the [https://2009.igem.org/Team:Imperial_College_London/Wetlab/Protocols/cellulase cellulase assay] and [https://2009.igem.org/Team:Imperial_College_London/Wetlab/Protocols/PAH PAH assay]. Enzyme activity is the rate of substrate utilisation /product formation per unit time. | ||
===Our Goals=== | ===Our Goals=== | ||
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This model aims to better understand the enzymatic action of the drug by: | This model aims to better understand the enzymatic action of the drug by: | ||
Revision as of 14:19, 18 September 2009
- Overview
- The model
- Simulations
Kinetics of protein drug
The protein drug of interest is an enzyme. It will bind to specific substrates and increase the rate of their conversion into products. Therefore, by monitoring either the substrate concentration or the product concentration, we can indirectly see the activity of the enzyme. This is quantified by measuring by enzyme activity in the cellulase assay and PAH assay. Enzyme activity is the rate of substrate utilisation /product formation per unit time.
Our Goals
This model aims to better understand the enzymatic action of the drug by:
- Characterizing its enzymatic activity
- Subsequently,by modeling the relationship between the quantity of protein being produced and the enzyme activity using a simple Michaelis-Menten enzyme kinetics model will enable us to take into account factors that could impair enzymatic activity
This model will allow an understanding of the activity of the enzymes and allow the WETLAB to have an idea of the magnitude of the enzymatic activity. More importantly, after the results from enzyme assays are obtained, the model should provide a means of relating the activity output data to the concentration of the enzyme.
