Template:Team:KULeuven/17 September 2009/BlueLightReceptor

From 2009.igem.org

(Difference between revisions)
(New page: # electroporation of logA (ligA in pBR322, a low copy plasmid). # a PCR on pSB3K3 with primers ...... # restriction digest of pSB3K3, pBR322 and ligA with EcoRI and PstI followed by a gel ...)
 
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# ligations of ligA (blp+GFP) with pBR322 (logA) and pSB3K3 (likA) were put overnight
# ligations of ligA (blp+GFP) with pBR322 (logA) and pSB3K3 (likA) were put overnight
# {{kulpart|BBa_I20260}} was taken from the distribution plates and plated on LB
# {{kulpart|BBa_I20260}} was taken from the distribution plates and plated on LB
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# liquid cultures of ligY (blp) were made
+
# liquid cultures of ligY (blp) were made in the morning, grew and were miniprepped in the evening followed by a restriction digest with EcoRI and PstI
-
# restriction digest of ligY with EcoRI and PstI
+

Latest revision as of 09:21, 21 September 2009

  1. electroporation of logA (ligA in pBR322, a low copy plasmid).
  2. a PCR on pSB3K3 with primers ......
  3. restriction digest of pSB3K3, pBR322 and ligA with EcoRI and PstI followed by a gel electrophoresis and extraction.
  4. ligations of ligA (blp+GFP) with pBR322 (logA) and pSB3K3 (likA) were put overnight
  5. was taken from the distribution plates and plated on LB
  6. liquid cultures of ligY (blp) were made in the morning, grew and were miniprepped in the evening followed by a restriction digest with EcoRI and PstI
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