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Team:Chiba/Notebook/Calendar/9 September 2009
From 2009.igem.org
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:*divided injection 12.5ml of the culture to 10 50ml centrifuging tubes | :*divided injection 12.5ml of the culture to 10 50ml centrifuging tubes | ||
:*WASH 3 times | :*WASH 3 times | ||
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:*after all, mix the supernatant and solid media at 1:1 | :*after all, mix the supernatant and solid media at 1:1 | ||
:*make 10 concentration varieties of solid media | :*make 10 concentration varieties of solid media | ||
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== Lab Notebook == | == Lab Notebook == | ||
[[Image:Chiba_Labwork_09Sep09.jpg|200px]] [[Image:Chiba_Labwork_09Sep09-2.jpg|200px]] | [[Image:Chiba_Labwork_09Sep09.jpg|200px]] [[Image:Chiba_Labwork_09Sep09-2.jpg|200px]] | ||
Revision as of 18:06, 23 September 2009
(8_September_2009 <|>10_September_2009)
08:00 main culture
- divided injection 12.5ml of the culture to 10 50ml centrifuging tubes
- WASH 3 times
- Add 12.5ml new culture
- Centrifuge the 1st tube 3000rpm 5min 20℃ and pick the upernatant
- Do same operation every 15min to 2nd~10th tubes
- after all, mix the supernatant and solid media at 1:1
- make 10 concentration varieties of solid media
Lab Notebook
