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Team:Chiba/Notebook/Calendar/8 September 2009
From 2009.igem.org
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Image:Chiba_0709_1945_1000_nM.jpg|1000 nM | Image:Chiba_0709_1945_1000_nM.jpg|1000 nM | ||
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| + | == Check of Sender's AHL generation (1) Day 1 == | ||
| + | ==== 20:45 start prior culture ==== | ||
| + | We did prior culture using glycerol stock(XL10G, plac-LuxI, PSB1, Amp) . | ||
== Handwritten Lab Notebook == | == Handwritten Lab Notebook == | ||
[[Image:Chiba_Labwork_08Sep09.jpg|200px]] | [[Image:Chiba_Labwork_08Sep09.jpg|200px]] | ||
Revision as of 18:19, 23 September 2009
(7_September_2009 <|>9_September_2009)
Contents |
Measurement of Receiver's Responce by AHL concentration (1)
- Inoculation
13:55
We cultured a sample at 37 degrees Celsius.
16:15
We transferred cultured sample into plate.
Observation of GFP fluorescence
We observed samples every 30 min. Here are pictures of samples.
16:15 (0)
 0 nM |
 1 nM |
 10 nM |
 100 nM |
 1000 nM |
 All |
16:45 (1)
 0 nM |
 1 nM |
 10 nM |
 100 nM |
 1000 nM |
17:15 (2)
 0 nM |
 1 nM |
 10 nM |
 100 nM |
 1000 nM |
 All |
17:45 (3)
 0 nM |
 1 nM |
 10 nM |
 100 nM |
 1000 nM |
 All |
18:15 (4)
 0 nM |
 1 nM |
 10 nM |
 100 nM |
 1000 nM |
 All |
18:45 (5)
 0 nM |
 1 nM |
 10 nM |
 100 nM |
 1000 nM |
 All |
19:15 (6)
 0 nM |
 1 nM |
 10 nM |
 100 nM |
 1000 nM |
 All |
19:45 (7)
 0 nM |
 1 nM |
 10 nM |
 100 nM |
 1000 nM |
Check of Sender's AHL generation (1) Day 1
20:45 start prior culture
We did prior culture using glycerol stock(XL10G, plac-LuxI, PSB1, Amp) .
Handwritten Lab Notebook
