"
Team:Imperial College London/Wetlab/Protocols/Cellculture/LBPlates
From 2009.igem.org
(Difference between revisions)
(→Make LB agar plates + antibiotic) |
(→Day 2) |
||
| Line 17: | Line 17: | ||
<li>Microwave LB agar flask until agar dissolves</li> | <li>Microwave LB agar flask until agar dissolves</li> | ||
<li>Put the LB agar flask into the water bath for 20-30 mins (this ensures the agar is dissolved properly)</li> | <li>Put the LB agar flask into the water bath for 20-30 mins (this ensures the agar is dissolved properly)</li> | ||
| - | <li>Turn on the Bunsen burner for sterile technique</li> | + | <li>Turn on the Bunsen burner. From now on work next to the flame for sterile technique.</li> |
<li>5 minutes before pipetting (see next step), take antibiotics out of the freezer and let them defrost</li> | <li>5 minutes before pipetting (see next step), take antibiotics out of the freezer and let them defrost</li> | ||
<li>When antibiotics have defrosted, add 300μL of antibiotic to 300 mL of LB agar. Ratio of AB:LB is 1:1000</li> | <li>When antibiotics have defrosted, add 300μL of antibiotic to 300 mL of LB agar. Ratio of AB:LB is 1:1000</li> | ||
Revision as of 12:14, 24 September 2009
Make LB agar plates + antibiotic
Day 1
- Make LB agar [[|How to Link]]
- Send LB agar for autoclaving (before 12.30)
Day 2
- Get LB agar from autoclave
- Switch water bath to 50C
- Microwave LB agar flask until agar dissolves
- Put the LB agar flask into the water bath for 20-30 mins (this ensures the agar is dissolved properly)
- Turn on the Bunsen burner. From now on work next to the flame for sterile technique.
- 5 minutes before pipetting (see next step), take antibiotics out of the freezer and let them defrost
- When antibiotics have defrosted, add 300μL of antibiotic to 300 mL of LB agar. Ratio of AB:LB is 1:1000
