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August/24 August 2009
From 2009.igem.org
(Difference between revisions)
| Line 55: | Line 55: | ||
↓<br> | ↓<br> | ||
gel electrophoresis<br> | gel electrophoresis<br> | ||
| + | [[Image:Osaka090824.jpg]] | ||
| - | + | result | |
| - | result | + | |
| - | + | 7 (RBS+cinR) | |
| - | 7 (RBS+cinR) | + | |
7-1 OK | 7-1 OK | ||
| - | + | ||
| - | 8 (RBS+LuxI) | + | 8 (RBS+LuxI) |
8-1 OK | 8-1 OK | ||
| - | + | ||
| - | 9 (RBS+LasR+terminater) | + | 9 (RBS+LasR+terminater) |
9 fialure? | 9 fialure? | ||
| - | + | ||
| - | 10 (RBS+EspE+terminater) | + | 10 (RBS+EspE+terminater) |
10 ??? | 10 ??? | ||
| - | + | ||
| - | 11(p(tetR)+RBS+GFP+terminater) | + | 11(p(tetR)+RBS+GFP+terminater) |
11-B OK | 11-B OK | ||
No. 9 10 → ligation rapid check | No. 9 10 → ligation rapid check | ||
Revision as of 09:26, 26 September 2009
1. LB+Amp plate
2.Min prep
Result of Nanodrop concentration check
(sample No.) (concentration)
7-1 27.8 ng/uL
8-1 23.2
9 21.1
10-A 29.1
10-B 25.1
11-A 15.7
11-B 34.1
3.transformed check
Checked the cell cultures transformed and plated out yesterday (8/23) for colony formation and made an approximate count of the number of colonies.
(sample No.) (no. of colonies)
1-4E ~5
1-8E 0
2-16L 1
2-16N ~15
2-18H 2
ligation sample
12 0
13 0
14 0
15 0
5 0
to iquid medium(5mL + Amp 5uL)
4.ligation check
| sample | 5 |
| XbaI | 1 |
| PstI | 1 |
| No.2 | 2 |
| dH2O | 11 |
| total | 20uL |
↓
37 , 2hr
↓
gel electrophoresis
result 7 (RBS+cinR) 7-1 OK 8 (RBS+LuxI) 8-1 OK 9 (RBS+LasR+terminater) 9 fialure? 10 (RBS+EspE+terminater) 10 ??? 11(p(tetR)+RBS+GFP+terminater) 11-B OK
No. 9 10 → ligation rapid check
