Team:UNICAMP-Brazil/Notebooks/September 21
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Revision as of 19:42, 26 September 2009
ColiGuard
Digested F plasmid's electroelution
- The digested F plasmid (digested with HindIII) was electroeluted, according to protocol 9. A 110 V voltage was applied during 3h before its extraction from the membrane.
- The next step is to verify if the electroelution went OK. The collected samples of purified plasmid will be applied to a 0,8% agarose gel.
Gabriel