August/24 August 2009

From 2009.igem.org

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Revision as of 09:34, 29 September 2009

1. LB+Amp plate

2.Min prep
Result of Nanodrop concentration check

(sample No.) (concentration)
     7-1          27.8   ng/uL
     8-1          23.2
      
     9             21.1
     10-A         29.1
     10-B         25.1
     11-A         15.7
     11-B         34.1

3.transformed check
Checked the cell cultures transformed and plated out yesterday (8/23) for colony formation and made an approximate count of the number of colonies.

(sample No.) (no. of colonies)          
    [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0051 1-4E]            ~5
    [http://partsregistry.org/wiki/index.php?title=Part:BBa_R1051 1-8E]             0
    [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118005 2-16L]            1
    [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118006 2-16N]           ~15
    [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118014 2-18H]            2
    
  ligation sample 
      12             0
      13             0
      14             0
      15             0
      
       5             0

 to iquid medium(5mL + Amp 5uL)

4.ligation check

sample	5
XbaI	1
PstI	1
No.2	2
dH₂O	11
total	20uL

↓
37 , 2hr
↓
gel electrophoresis

result

7 (RBS+cinR)
7-1     OK

8 (RBS+LuxI)
8-1     OK

9 (RBS+LasR+terminater)
9       fialure?

10 (RBS+EspE+terminater)
10     ???

11(p(tetR)+RBS+GFP+terminater)
11-B    OK

No. 9 10 → ligation rapid check