Team:Imperial College London/Wetlab/Protocols/GenomePrep

From 2009.igem.org

(Difference between revisions)
Droche (Talk | contribs)
(New page: {{Imperial/09/TemplateTop}} === Restriction Protocol === <br> Perform for Dam +ve, -ve and TOP10 Strains In a PCR tube, mix up the following: <br> * 5ul Genome DNA from Prep * 2ul of H...)
Newer edit →

Revision as of 10:30, 30 September 2009


Restriction Protocol


Perform for Dam +ve, -ve and TOP10 Strains In a PCR tube, mix up the following:

  • 5ul Genome DNA from Prep
  • 2ul of H2O
  • 1ul of 10X BSA
  • 1ul of 10X B2
  • 0.5ul of TaqI
  • 0.5ul of DpnII

Mr. Gene   Geneart   Clontech   Giant Microbes

Retrieved from "http://2009.igem.org/Team:Imperial_College_London/Wetlab/Protocols/GenomePrep"