Imperial College London/Notebook/1 October 2009

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===Secondary carbon sources===
===Secondary carbon sources===
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*Running as we speak
 +
===GFP pH===
===GFP pH===
===IPTG growth===
===IPTG growth===
{{Imperial/09/TemplateBottom}}
{{Imperial/09/TemplateBottom}}

Revision as of 15:28, 1 October 2009

Contents

Thursday the 1st of October

Testing team (update)

Harvard-GFP

  • There is a difference in fluorescence between 28°C and 37°C after compensating for OD.
  • Need to adjust the gain so that fluorescence doesn't saturate
  • Runs: Wed 23rd September, 30th

OD calibration

  • 17th of September
  • 23rd of September
  • 25th of September
  • Preliminary results show that cell number varies with OD, repetition of the experiment will allow to fit a curve to the graph: cell number vs OD.

Secondary carbon sources

  • Running as we speak

GFP pH

IPTG growth

Mr. Gene   Geneart   Clontech   Giant Microbes

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