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Imperial College London/Notebook/11 September 2009
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====Protocol considerations==== | ====Protocol considerations==== | ||
| - | Using spectrophotometer at 600nm | + | *Using spectrophotometer at 600nm |
| - | Blanks are all the relevant carbon sources, but subsequently realised that all blanks were roughly the same (within 0.03) | + | *Blanks are all the relevant carbon sources, but subsequently realised that all blanks were roughly the same (within 0.03) |
| - | Therefore, subsequent readings= blank+ 6 sample | + | **Therefore, subsequent readings= blank + 6 sample |
| - | Overnight culture had OD of 1.64. | + | *Overnight culture had OD of 1.64. |
| - | 800ul was put into each sample of 40ml~ 0.033 OD | + | **800ul was put into each sample of 40ml~ 0.033 OD |
| - | ie same initial OD | + | **ie same initial OD |
| - | Grown in 6 separate 50ml falcon tubes and shaking in incubator at 28 degrees Celsius | + | *Grown in 6 separate 50ml falcon tubes and shaking in incubator at 28 degrees Celsius |
| - | 1ml taken out at 1 hr intervals from the falcon tube and placed into spectrophotometer | + | *1ml taken out at 1 hr intervals from the falcon tube and placed into spectrophotometer |
====Results==== | ====Results==== | ||
Revision as of 12:18, 2 October 2009
Contents |
Testing
Diauxic growth 11th Sep
Protocol considerations
- Using spectrophotometer at 600nm
- Blanks are all the relevant carbon sources, but subsequently realised that all blanks were roughly the same (within 0.03)
- Therefore, subsequent readings= blank + 6 sample
- Overnight culture had OD of 1.64.
- 800ul was put into each sample of 40ml~ 0.033 OD
- ie same initial OD
- Grown in 6 separate 50ml falcon tubes and shaking in incubator at 28 degrees Celsius
- 1ml taken out at 1 hr intervals from the falcon tube and placed into spectrophotometer
Results
Conclusions
Suggestions/Improvements
