Lé Protocols

Primer PCR protocol - We used it for: Making a full RIP molecule from our oligos.

Purification protocol from gel - We used it for: Gel purification, over and over and over.

Purification protocol from solution - We used it for: PCR product purification.

Making cells competent - We used it for: Making competent cells for both transformation and electroporation.

Transformation - We used it for: Transforming plasmids (containing biobricks) into competent cells.

Electroporation - We used it for: Transforming plasmids (containing biobricks) into competent cells. Electroporation is our preferred technique, since it requires less plasmid volume and uses electricity!

Miniprep - We used it for: Purifying plasmids from inoculations of transformed bacteria.

How to make an agarose gel - We used it for: Making Agarose gels, for use in testing brick and plasmid sizes. Very central technique to controlling your lab work.

Restrictions - We used it for: Cutting out biobricks and preparing backbones for insertion of biobricks.

Ligations - We used it for: Pasting our biobricks into backbones.