Team:Freiburg bioware/Notebook/April

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===29th april 21o'clock Rüdiger===
===29th april 21o'clock Rüdiger===
<table>
<table>
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<tr><td><img src="Freiburg09_huebsche_fokI.jpg"Figure 1: FokI-Model, ribbon-style. DNA Strand is bound by two FOKI|400x400px]]</td>
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<tr><td><img src="Freiburg09_huebsche_fokI.jpg" name="Figure 1: FokI-Model, ribbon-style. DNA Strand is bound by two FOKI" width="400" height="400" alt="FokI bound to dsDNA" /></td>
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<td>[[Image:freiburg09_huebsche fokI_surface_render.jpg|none|thumb|Figure 2: FokI-Model surface rendering. DNA Strand is bound by two FOKI|400x400px]]</td></tr>
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<td>[[Image:freiburg09_huebsche fokI_surface_render.jpg|none|thumb|Figure 2: FokI-Model surface rendering. DNA Strand is bound by two FOKI width="400" height="400"></td></tr>
<tr><td>[[Image:freiburg09_fokI_3oligos.JPG|none|thumb|Schema 3: 2Oligos, both with active fokI, one cutting DNA, the other cutting a third oligo|400x400px]]</td>
<tr><td>[[Image:freiburg09_fokI_3oligos.JPG|none|thumb|Schema 3: 2Oligos, both with active fokI, one cutting DNA, the other cutting a third oligo|400x400px]]</td>
<td>[[Image:freiburg09_fokI_1inactive.JPG|none|thumb|Schema 4: 2Oligos, one with active fokI cutting DNA, the other inactive, just binding its own oligo|400x400px]]</td></tr>
<td>[[Image:freiburg09_fokI_1inactive.JPG|none|thumb|Schema 4: 2Oligos, one with active fokI cutting DNA, the other inactive, just binding its own oligo|400x400px]]</td></tr>

Revision as of 07:35, 6 October 2009

FREiGEM 2009

April


===24th april 12:30 - 18:00 o'clock Manuel=== Literaturrecherche zu FokI.
Entstandene Ideen:
  • drei Oligo's: zwei für die Bindung der Monomere und eines zur Erzeugung der dsDNA an die FokI binden kann.
  • zwei Oligo's: eines zur Bindung des einen Monomers, das zweite zur Bindung des zweiten Monomers und zur Ausbildung der dsDNA (ein Monomer müsste hierfür inaktiviert werden).
  • ein Oligo: beide Bindestellen der Monomere auf einem Oligo. Auch hier müsste ein Monomer inaktiviert sein.
    • Es wurde die 196 C-terminalen Aminosäuren von FokI verwendet. Primersequenzen sind vorhanden.
    [[Media: Freiburg09_FokI_Zn-Finger_1996.pdf]]


    ===29th april 21o'clock Rüdiger===
    FokI bound to dsDNA [[Image:freiburg09_huebsche fokI_surface_render.jpg|none|thumb|Figure 2: FokI-Model surface rendering. DNA Strand is bound by two FOKI width="400" height="400">
    [[Image:freiburg09_fokI_3oligos.JPG|none|thumb|Schema 3: 2Oligos, both with active fokI, one cutting DNA, the other cutting a third oligo|400x400px]] [[Image:freiburg09_fokI_1inactive.JPG|none|thumb|Schema 4: 2Oligos, one with active fokI cutting DNA, the other inactive, just binding its own oligo|400x400px]]
    [[Image:freiburg09_fokI_1oligo.JPG|none|thumb|Schema 5: 1Oligo with active FokI cutting DNA, the other inactive, just binding its own oligo|400x400px]] [[Image:freiburg09_fokI_pna.JPG|none|thumb|Schema 6: 2Oligo, with active fokI cutting DNA, attached on both FokI is a specific PNA. Oligos must be designed to bind this sequence. It is to be discussed whether there is a third oligo required for 3d stabilisation of dna |400x400px]]







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