From 2009.igem.org
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Revision as of 08:54, 6 October 2009
iGEM Team:ULB-Brussels Wiki
| July
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| M | T | W | T | F | S | S
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| [http://2009.igem.org/ULB/1_July_2009 1]
| [http://2009.igem.org/ULB/2_July_2009 2]
| [http://2009.igem.org/wiki/index.php?title=ULB/3_July_2009&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=ULB/4_July_2009&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=ULB/5_July_2009&action=edit 5]
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| [http://2009.igem.org/wiki/index.php?title=ULB/6_July_2009&action=edit 6]
| [http://2009.igem.org/ULB/7_July_2009 7]
| [http://2009.igem.org/wiki/index.php?title=ULB/8_July_2009&action=edit 8]
| [http://2009.igem.org/ULB/9_July_2009 9]
| [http://2009.igem.org/wiki/index.php?title=ULB/10_July_2009&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=ULB/11_July_2009&action=edit 11]
| [http://2009.igem.org/ULB/12_July_2009 12]
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| [http://2009.igem.org/wiki/index.php?title=ULB/13_July_2009&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=ULB/14_July_2009&action=edit 14]
| [http://2009.igem.org/ULB/15_July_2009 15]
| [http://2009.igem.org/wiki/index.php?title=ULB/16_July_2009&action=edit 16]
| [http://2009.igem.org/wiki/index.php?title=ULB/17_July_2009&action=edit 17]
| [http://2009.igem.org/wiki/index.php?title=ULB/18_July_2009&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=ULB/19_July_2009&action=edit 19]
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| [http://2009.igem.org/ULB/20_July_2009 20]
| [http://2009.igem.org/ULB/21_July_2009 21]
| [http://2009.igem.org/ULB/22_July_2009 22]
| [http://2009.igem.org/ULB/23_July_2009 23]
| [http://2009.igem.org/ULB/24_July_2009 24]
| [http://2009.igem.org/ULB/25_July_2009 25]
| [http://2009.igem.org/wiki/index.php?title=ULB/26_July_2009&action=edit 26]
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| [http://2009.igem.org/wiki/index.php?title=ULB/27_July_2009&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=ULB/28_July_2009&action=edit 28]
| [http://2009.igem.org/ULB/29_July_2009 29]
| [http://2009.igem.org/wiki/index.php?title=ULB/30_July_2009&action=edit 30]
| [http://2009.igem.org/wiki/index.php?title=ULB/31_July_2009&action=edit 31]
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| August
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| M | T | W | T | F | S | S
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| [http://2009.igem.org/wiki/index.php?title=ULB/1_August_2009&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=ULB/2_August_2009&action=edit 2]
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| [http://2009.igem.org/ULB/3_August_2009 3]
| [http://2009.igem.org/ULB/4_August_2009 4]
| [http://2009.igem.org/wiki/index.php?title=ULB/5_August_2009&action=edit 5]
| [http://2009.igem.org/ULB/6_August_2009 6]
| [http://2009.igem.org/ULB/7_August_2009 7]
| [http://2009.igem.org/wiki/index.php?title=ULB/8_August_2009&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=ULB/9_August_2009&action=edit 9]
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| [http://2009.igem.org/wiki/index.php?title=ULB/10_August_2009&action=edit 10]
| [http://2009.igem.org/ULB/11_August_2009 11]
| [http://2009.igem.org/ULB/12_August_2009 12]
| [http://2009.igem.org/ULB/13_August_2009 13]
| [http://2009.igem.org/ULB/14_August_2009 14]
| [http://2009.igem.org/wiki/index.php?title=ULB/15_August_2009&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=ULB/16_August_2009&action=edit 16]
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| [http://2009.igem.org/ULB/17_August_2009 17]
| [http://2009.igem.org/ULB/18_August_2009 18]
| [http://2009.igem.org/ULB/19_August_2009 19]
| [http://2009.igem.org/ULB/20_August_2009 20]
| [http://2009.igem.org/ULB/21_August_2009 21]
| [http://2009.igem.org/wiki/index.php?title=ULB/22_August_2009&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=ULB/23_August_2009&action=edit 23]
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| [http://2009.igem.org/wiki/index.php?title=ULB/24_August_2009&action=edit 24]
| [http://2009.igem.org/ULB/25_August_2009 25]
| [http://2009.igem.org/ULB/26_August_2009 26]
| [http://2009.igem.org/wiki/index.php?title=ULB/27_August_2009&action=edit 27]
| [http://2009.igem.org/ULB/28_August_2009 28]
| [http://2009.igem.org/wiki/index.php?title=ULB/29_August_2009&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=ULB/30_August_2009&action=edit 30]
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| [http://2009.igem.org/ULB/31_August_2009 31]
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| September
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| M | T | W | T | F | S | S
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| [http://2009.igem.org/ULB/1_September_2009 1]
| [http://2009.igem.org/wiki/index.php?title=ULB/2_September_2009&action=edit 2]
| [http://2009.igem.org/ULB/3_September_2009 3]
| [http://2009.igem.org/ULB/4_September_2009 4]
| [http://2009.igem.org/ULB/5_September_2009 5]
| [http://2009.igem.org/wiki/index.php?title=ULB/6_September_2009&action=edit 6]
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| [http://2009.igem.org/ULB/7_September_2009 7]
| [http://2009.igem.org/wiki/index.php?title=ULB/8_September_2009&action=edit 8]
| [http://2009.igem.org/ULB/9_September_2009 9]
| [http://2009.igem.org/ULB/10_September_2009 10]
| [http://2009.igem.org/ULB/11_September_2009 11]
| [http://2009.igem.org/ULB/12_September_2009 12]
| [http://2009.igem.org/wiki/index.php?title=ULB/13_September_2009&action=edit 13]
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| [http://2009.igem.org/ULB/14_September_2009 14]
| [http://2009.igem.org/wiki/index.php?title=ULB/15_September_2009&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=ULB/16_September_2009&action=edit 16]
| [http://2009.igem.org/wiki/index.php?title=ULB/17_September_2009&action=edit 17]
| [http://2009.igem.org/wiki/index.php?title=ULB/18_September_2009&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=ULB/19_September_2009&action=edit 19]
| [http://2009.igem.org/wiki/index.php?title=ULB/20_September_2009&action=edit 20]
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| [http://2009.igem.org/wiki/index.php?title=ULB/21_September_2009&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=ULB/22_September_2009&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=ULB/23_September_2009&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=ULB/24_September_2009&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=ULB/25_September_2009&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=ULB/26_September_2009&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=ULB/27_September_2009&action=edit 27]
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| [http://2009.igem.org/wiki/index.php?title=ULB/28_September_2009&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=ULB/29_September_2009&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=ULB/30_September_2009&action=edit 30]
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July 12, 2009
Forward and reverse primers are designed and ordered.
Primers design for hfsG et hfsH genes
BBa_R0011 (promoter) :
PREFIX :
=> GAATTCATGAGATCT aattgtgagcggataaca
SUFFIX :
<= ctcgagttaggatcc TGTGCTCAGTATCTTGTT
RBS BBa_B0034 :
PREFIX :
=> GAATTCATGAGATCT AAAGAG
SUFFIX :
<= ctcgagttaggatcc TTTCTC
hfsG gene :
PREFIX :
=> GAATTCATGAGATCT atgaacgcgcccgtcaacgagctgcgactggagaacgccg
SUFFIX :
<= cagacggcctcgctgtaga
hfsH gene :
PREFIX :
=> atgccgatggagttcgagaag
SUFFIX :
<= ctcgagttaggatcc ttagagcccgatcc
RBS + RFP :
PREFIX :
=> GAATTCATGAGATCT aaagaggagaaatactag
SUFFIX :
<= ctcgagttaggatcc GCGATCTACACTAGCACT
BBa_B0015 terminator :
PREFIX :
=> GAATTCATGAGATCT ccaggcatcaaataaaac
SUFFIX :
<= ctcgagttaggatcc TATAAACGCAGAAAGGCC
Sequences
Promoter (55 bp)
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
RBS (12 pb)
aaagaggagaaa
RBS + RFP (681 bp)
aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgt
atggaaggttccgttaacggtcacgagttcgAaatcgaaggtgaaggtgaaggtcgtccgtacgaaggt
acccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccg
cagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtcc
ttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag
gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgac
ggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggt
gctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaa
accacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatc
acctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggt
gcttaataacgctgatagtgctagtgtagatcgc
Terminator (129 bp)
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
Plasmid (3165 bp)
ggatcctaactcgaggttacattgtcgatctgttcatggtgaacagctttaaatgcaccaaaaactcgt
aaaagctctgatgtatctatcttttttacaccgttttcatctgtgcatatggacagttttccctttgat
atctaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagcc
ataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgag
ccatgagaacgaaccattgagatcatgcttactttgcatgtcactcaaaaattttgcctcaaaactggt
gagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttacgtaggtaggaatctga
tgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatc
catttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaa
tttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaa
ctcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgcctt
gtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaa
agacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttc
actaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcc
tttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacacc
ataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttc
tttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatg
ctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatct
caattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattacatgtcct
tttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctag
accctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatag
aataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtc
agttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccc
taaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatca
ggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggg
gtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccg
tcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagc
agttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggct
aatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtccctagtgcttggattc
tcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcatt
actggatctatcaacaggagtccaagcgagctcgtaaacttggtctgacagttaccaatgcttaatcag
tgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagat
aactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcacc
ggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaacttt
atccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagttt
gcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcag
ctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctcctt
cggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgca
taattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcatt
ctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccaca
tagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttacc
gctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcac
cagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaa
atgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgag
cggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttcccatggtgcc
acctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaa
tttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcatgagatct
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