Team:Imperial College London/M3/DamMethylation
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Revision as of 20:37, 6 October 2009
- Overview
- Restriction Enzymes
- Dam Methylation
- Thermoinduction
Dam methylation
To protect against DNA destruction due to basal levels of restriction enzyme production, we have made use of the native E. coli Dam methylase protection system. This methylates DNA. Therefore, only high levels of restriction enzyme (ie. after thermal triggering) will cleave the DNA.