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Team:Imperial College London/M1/Genetic
From 2009.igem.org
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To start the drug manufacturing process, IPTG is pipetted into the system. IPTG will repress LacI. As a result, protein production is de-repressed. Then, the enzymes PAH or cellulase will be produced by the <i>E.ncapsulator</i>. | To start the drug manufacturing process, IPTG is pipetted into the system. IPTG will repress LacI. As a result, protein production is de-repressed. Then, the enzymes PAH or cellulase will be produced by the <i>E.ncapsulator</i>. | ||
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| + | {{Imperial/09/Division}} | ||
| + | <center><b>Module 1: Enzyme Production</b></center> | ||
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| + | <html><center><a href="https://2009.igem.org/Team:Imperial_College_London/M1"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Homepageimage3.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/M1/EnzymeDelivery"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Homepageimage3.png"></a><a | ||
| + | href="https://2009.igem.org/Team:Imperial_College_London/M1/PeptideDelivery"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Homepageimage3.png"></a><a | ||
| + | href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/M2/Wetlab"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Homepageimage3.png"></a><html><a | ||
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| + | href="https://2009.igem.org/Team:Imperial_College_London/M2/Modelling"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Homepageimage3.png"></a><center></html> | ||
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| + | <html><table border="0" style="background-color:transparent;" width="100%"> | ||
| + | <tr><td width="0%"></td> | ||
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| + | <td width="20%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/M1"><b>Module 1 Overview</b></a></center></td> | ||
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| + | <td width="20%"><center><a href="/Team:Imperial_College_London/M1/Enzyme Delivery"><b>Enzyme Delivery </b></a></center></td> | ||
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| + | <td width="20%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/M1/PeptideDelivery"><b>Peptide Delivery</b></a></center></td> | ||
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| + | <td width="20%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/M2/Wetlab"><b>WetLab</b></a></center></td> | ||
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| + | <td width="20%"><center><a | ||
| + | href="https://2009.igem.org/Team:Imperial_College_London/M2/Modelling"><b>Modelling</b></a></center></td> | ||
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| + | <td width="1%"></td> | ||
| + | </tr></table></html> | ||
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{{Imperial/09/TemplateBottom}} | {{Imperial/09/TemplateBottom}} | ||
Revision as of 13:36, 8 October 2009
M1 Genetic Circuit
This is the genetic circuit responsible protein drug fabrication (Module 1).
LacI is produced constitutively by the E. coli bacterium, and it represses the pLambda promoter. This inhibits production of the proteins of interest (PAH and cellulase).
To start the drug manufacturing process, IPTG is pipetted into the system. IPTG will repress LacI. As a result, protein production is de-repressed. Then, the enzymes PAH or cellulase will be produced by the E.ncapsulator.
