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Team:Valencia/Parts/Characterization
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[[Image:aequorin.GIF|200px|center]] | [[Image:aequorin.GIF|200px|center]] | ||
| - | It has been introduced in our yeasts by a plasmid called pEVP11/AEQ, wich encondes aequorin sequence showed below. Cells containing this plasmid are able to sintetize aequorin and keep it in their citoplasm. So, that protein can't produce luminiscence by itself, but | + | It has been introduced in our yeasts by a plasmid called pEVP11/AEQ, wich encondes aequorin sequence showed below. Cells containing this plasmid are able to sintetize aequorin and keep it in their citoplasm. So, that protein can't produce luminiscence by itself, but when it binds to its cofactor coelenterazine, in presence of Ca2+, aequorin emits light. |
==Sequence== | ==Sequence== | ||
Revision as of 11:57, 9 October 2009
General information
AEQ is a gene which encodes for aequorin, our luminiscent protein.
It has been introduced in our yeasts by a plasmid called pEVP11/AEQ, wich encondes aequorin sequence showed below. Cells containing this plasmid are able to sintetize aequorin and keep it in their citoplasm. So, that protein can't produce luminiscence by itself, but when it binds to its cofactor coelenterazine, in presence of Ca2+, aequorin emits light.
Sequence
Aequorin sequence is (primer binding sites are underlined in green):
