Team:Imperial College London/M2/genes

Colanic Acid Biosynthesis:

wcaF

• Putative colanic acid biosynthesis acetyltransferase ²

gmd

Codes for GDP-mannose 4,6-dehydratase which is a subunit of GDP-mannose 4,6-dehydratase. This enzyme is involved in colanic acid biosynthesis.

wcaD

• Predicted colanic acid polymerase

wza

• This codes for a lipoprotein that is required for colanic acid translocation through the outer membrane. The lipoprotein is a subunit of the capsular polysaccharide export apparatus.

fcl

• Codes for a subunit of GDP-fucose synthase. This enzyme is involved in colanic acid biosynthesis.

General Protective:

ivy (Inhibitor of Vertebrate lysozyme)

• Discovered in 2001 as the first bacterial lysozyme inhibitor. This Type-C lysozyme inhibitor resides in the periplasm.¹

MilC (Membrane-bound lysozyme inhibitor of Type C lysozyme)

• This is a lipoprotein that resides in the membrane. ¹

OsmC (Osmotically-inducible protein C)

• This protein provides defense against oxidative stress caused by exposure to organic hydroperoxides. ^{2 3}

KatE

• Codes for a subunit of heme d synthase / hydroperoxidase. This bacterial catalase protects against osmotic stress.

Global Regulators:

Sra (stationary-phase-induced ribosome-associated)

Sra is a sub-stoichiometric component of the 30S ribosomal subunit that is more abundant at stationary phase than during log phase growth.

rprA

rprA is a gene encoding a small RNA molecule that is involved in the post-transcriptional control of the alternative sigma factor, ("sigma S").

FtsA

FtsA is an essential cell division protein which colocalizes with FtsZ to the septal ring structure.

FtsZ

• Essential cell division protein.

yfbR

• dCMP phosphohydrolase

Membrane Proteins:

bdm (biofilm-dependent modulation protein)

OsmB

• OsmB encodes an outer membrane lipoprotein.

OsmY

Hyperosmotically inducible periplasmic protein

PhnQ

ycfJ

• Uncharacterized putative inner membrane protein.

ygaC

spy

ymgD

• Putative periplasmic protein.

ypeC

• Unknown function

ymgG

YbaY

Predicted outer membrane lipoprotein

References

1) [http://www.ncbi.nlm.nih.gov/pubmed/19136591 The Rcs two-component system regulates expression of lysozyme inhibitors and is induced by exposure to lysozyme]

2) [http://www.ncbi.nlm.nih.gov/pubmed/19346340 Gene Expression Induced in Escherichia coli O157:H7 upon Exposure to Model Apple Juice]

3) [http://www.ncbi.nlm.nih.gov/pubmed/14627744 Structural and functional features of the Escherichia coli hydroperoxide resistance protein OsmC.]

4) [http://www.ncbi.nlm.nih.gov/pubmed/16153174?ordinalpos=5&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum The Rcs phosphorelay: a complex signal transduction system.]