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August/29 August 2009
From 2009.igem.org
(Difference between revisions)
(New page: 1. Colony check of cultures transformed on 8/28. Results were as follows: Part label or Plate location Approx. number of colonies (12) 10...) |
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1-6I over 100 | 1-6I over 100 | ||
| - | Plates (18), (20) and (21) were returned to incubator (37 degrees) to check for colony growth on the following day. | + | Plates (18), (20) and (21) were returned to incubator (37 degrees) to check for colony growth on the following day.<br> |
| - | 2. (12) to (16), (18), (19), and 1-6I were inoculated into growth culture solution. Miniprep should be conducted on the resulting cell cultures tomorrow. | + | 2. (12) to (16), (18), (19), and 1-6I were inoculated into growth culture solution. Miniprep should be conducted on the resulting cell cultures tomorrow.<br> |
| - | 3. (12), (15), (16) were streaked on a culture plate together with 1-23L as reference vector. Also, (13), (14), (19) were streaked together with 1-2M as reference vector. Ligation check (by electrophoresis) should be conducted on these tomorrow. | + | 3. (12), (15), (16) were streaked on a culture plate together with 1-23L as reference vector. Also, (13), (14), (19) were streaked together with 1-2M as reference vector.<br> Ligation check (by electrophoresis) should be conducted on these tomorrow. |
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| + | [https://2009.igem.org/Team:Osaka/NOTES back to NOTES] | ||
Latest revision as of 08:18, 12 October 2009
1. Colony check of cultures transformed on 8/28. Results were as follows: Part label or Plate location Approx. number of colonies
(12) 10
(13) 20
(14) 20
(15) 20
(16) over 100
(18) 3
(19) 20
(20) 0
(21) 0
1-6I over 100
Plates (18), (20) and (21) were returned to incubator (37 degrees) to check for colony growth on the following day.
2. (12) to (16), (18), (19), and 1-6I were inoculated into growth culture solution. Miniprep should be conducted on the resulting cell cultures tomorrow.
3. (12), (15), (16) were streaked on a culture plate together with 1-23L as reference vector. Also, (13), (14), (19) were streaked together with 1-2M as reference vector.
Ligation check (by electrophoresis) should be conducted on these tomorrow.
