Team:Imperial College London/Wetlab/Results

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(Growth in the presence of Glucose only)
(Growth in the presence of Glucose only)
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Testing the effects of glucose concentration on cell growth: This experiment will be linked to diauxic growth.<br>
Testing the effects of glucose concentration on cell growth: This experiment will be linked to diauxic growth.<br>
<b>Rationale:</b>  
<b>Rationale:</b>  
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A cell culture in the presence of glucose grows exponentially. Once it uses up its resource, it stops growing and the population density saturates.<br>
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A cell culture in the presence of glucose grows exponentially. Once it uses up its resource, it stops growing and the population density saturates.<br>
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<b>Experiment:</b> 
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In a 96-well plate, initial concentrations of glucose ranging from 0.01% to 0.05% of the culture were studied. There are several possible effects that it could exert on cell growth and the hypotheses are stated below:<br>
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1.The lower the initial amount of glucose in the medium, the shorter the time taken for the population to reach saturation level.<br>
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2.The lower the initial amount of glucose in the medium, the lower the growth rate prior saturation level, but no delays on the time taken to reach saturation. <br>
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In addition, we also know that a lower amount of glucose means that the population will grow less and saturate at a smaller value.
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===Growth in the presence of Glucose + Xylose ===
===Growth in the presence of Glucose + Xylose ===

Revision as of 09:44, 12 October 2009



Major results are shown in this section; there's a brief description of each and you can click through to find more detail on them. For minor results, please refer to our Notebook.

Contents

Module 1

Module 2

Module 3

Genome Deletion

Temporal Control

Diauxic Growth

Growth in the presence of Glucose only

Testing the effects of glucose concentration on cell growth: This experiment will be linked to diauxic growth.
Rationale: A cell culture in the presence of glucose grows exponentially. Once it uses up its resource, it stops growing and the population density saturates.

Growth in the presence of Glucose + Xylose

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