Team:UNIPV-Pavia/Methods Materials/Measurement

From 2009.igem.org

(Difference between revisions)

Revision as of 22:09, 12 October 2009

Measurements

A preliminar study was done on the instrument TECAN Infinite F200, a multifunctional microplate filter-based reader described in Instruments, to understand the behaviour of the measurements of absorbance and fluorescence intensity in different situations.

The results of the experiments, grouped by argoments, are described here, if you need more informations about a single experiment, you can find the detailed protocol at [http://aimed11.unipv.it/iGEM2009 Download Protocols].

Studies:

ABSORBANCE:

FLUORESCENCE:

EVAPORATION:

The "frame effect" on the plate


	CONCLUSIONS: THE BEST EXPERIMENTAL SET UP

	• In long experiments do not use the well on the frame, but fill them with water or growth medium to maintain the temperature homogenous in the plate; • in long experiments cover the plate with the lid to limit the evaporation; • use the O.D. measurements to quantify bacterial growth, regardless of the volume in the well; • do not dispense water if you perform fluorescence measurements; • work with volumes not larger than 200 μl and prefer the M9 medium, if you perform fluorescence measurements; •

@@ Line 39: / Line 39: @@
 *[[Team:UNIPV-Pavia/Methods_Materials/Absorbance#Absorbance and water dispensation| Absorbance and water dispensation]]
 *[[Team:UNIPV-Pavia/Methods_Materials/Absorbance#Absorbance and volume|Absorbance and volume]]
-*[[Team:UNIPV-Pavia/Methods_Materials/Absorbance#Absorbance and dilutions in liquid growing medium|Absorbance and dilutions in liquid growing medium]]
+*[[Team:UNIPV-Pavia/Methods_Materials/Absorbance#Absorbance and dilutions in liquid growth medium|Absorbance and dilutions in liquid growth medium]]
 '''FLUORESCENCE''':
@@ Line 45: / Line 45: @@
 *[[Team:UNIPV-Pavia/Methods_Materials/Fluorescence#Fluorescence and dilutions in water|Fluorescence and dilutions in water]]
 *[[Team:UNIPV-Pavia/Methods_Materials/Fluorescence#Fluorescence and volume|Fluorescence and volume]]
-*[[Team:UNIPV-Pavia/Methods_Materials/Fluorescence#Fluorescence and dilutions in liquid growing medium|Fluorescence and dilutions in liquid growing medium]]
+*[[Team:UNIPV-Pavia/Methods_Materials/Fluorescence#Fluorescence and dilutions in liquid growth medium|Fluorescence and dilutions in liquid growth medium]]
 *[[Team:UNIPV-Pavia/Methods_Materials/Fluorescence#Temporal evolution of fluorescence measurements|Temporal evolution of fluorescence measurements]]
 *[[Team:UNIPV-Pavia/Methods_Materials/Fluorescence#Bleaching and autofluorescence|Bleaching and autofluorescence]]
@@ Line 67: / Line 67: @@
 <tr bgcolor="royalblue">
 <td><font color="white">
-• In long experiments do not use the well on the frame, but fill them with water or growing medium to maintain the temperature homogenous in the plate;<br><br>
+• In long experiments do not use the well on the frame, but fill them with water or growth medium to maintain the temperature homogenous in the plate;<br><br>
 • in long experiments cover the plate with the lid to limit the evaporation;<br><br>
@@ Line 75: / Line 75: @@
 • do not dispense water if you perform fluorescence measurements;<br><br>
-• work with volumes not larger than 200 if you perform fluorescence measurements and prefer the M9 medium;<br><br>
+• work with volumes not larger than 200 μl and prefer the M9 medium, if you perform fluorescence measurements;<br><br>
 •