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Team:UNIPV-Pavia/Methods Materials/Measurement
From 2009.igem.org
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| - | + | BEST EXPERIMENTAL SET UP AND MEASUREMENT METHODS | |
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| - | • In long experiments do not use the | + | • In long experiments do not use the wells on the frame, but fill them with water or growth medium to maintain the temperature homogenous in the plate;<br><br> |
| - | • in long experiments cover the plate with the lid to limit the evaporation;<br><br> | + | • in long experiments cover the plate with the lid to limit the evaporation phenomenon;<br><br> |
• use the O.D. measurements to quantify bacterial growth, regardless of the volume in the well;<br><br> | • use the O.D. measurements to quantify bacterial growth, regardless of the volume in the well;<br><br> | ||
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Revision as of 22:16, 12 October 2009
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Measurements |
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A preliminar study was done on the instrument TECAN Infinite F200, a multifunctional microplate filter-based reader described in Instruments, to understand the behaviour of the measurements of absorbance and fluorescence intensity in different situations. The results of the experiments, grouped by argoments, are described here, if you need more informations about a single experiment, you can find the detailed protocol at [http://aimed11.unipv.it/iGEM2009 Download Protocols].
ABSORBANCE:
FLUORESCENCE:
EVAPORATION:
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