Team:Freiburg bioware/Notebook/April

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<h2 class="art-PostHeaderIcon-wrapper">April<span
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<h2 class="art-PostHeaderIcon-wrapper"> &nbsp;<img
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April
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<h3>24th April 12:30 - 18:00 o'clock Manuel</h3>
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<a href="#24">24th April
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<div style="text-align: left;">Literaturrecherche zu FokI.<br />
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</a>
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<br>
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<a href="#29">29th April
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</a>
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<h3><a name="24"></a>24th April 12:30 - 18:00 o'clock Manuel</h3>
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Literaturrecherche zu FokI.<br>
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Entstandene Ideen:
Entstandene Ideen:
-
 
<ul>
<ul>
-
<li>three oligo's: two for binding the monomers and one to create a dsDNA where FokI can bind.</li>
+
  <li>three oligo's: two for binding the monomers and one to
-
<li>two oligo's: one for binding one monomer, the second for binding the second monomer and to create dsDNA (one monomer has to be inactivated).</li>
+
create a dsDNA where FokI can bind.</li>
-
<li>one oligo: both binding sites of the monomers on one oligo (also one monomer has to be inactivated).</li>
+
  <li>two oligo's: one for binding one monomer, the second for
 +
binding
 +
the second monomer and to create dsDNA (one monomer has to be
 +
inactivated).</li>
 +
  <li>one oligo: both binding sites of the monomers on one oligo
 +
(also one monomer has to be inactivated).</li>
</ul>
</ul>
-
<br>
+
<br />
-
The 196 C-terminale aminoacids of FokI were used from other groups (see reference 1). Primersequences are also provided in the publication.<br>
+
The 196 C-terminale aminoacids of FokI were used from other groups (see
-
 
+
reference 1). Primersequences are also provided in the publication.<br />
Reference 1: Freiburg09_FokI_Zn-Finger_1996.pdf
Reference 1: Freiburg09_FokI_Zn-Finger_1996.pdf
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<br><br>
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<h3><a name="29"></a>29th april 21o'clock
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<h3><a name="29"></a>29th april 21o'clock Rüdiger</h3>
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R&uuml;diger</h3>
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<td><img src="https://static.igem.org/mediawiki/2009/9/99/Freiburg09_huebsche_fokI.jpg" name="Figure 1: FokI-Model, ribbon-style. DNA Strand is bound by two FOKI" width="300" height="300" alt="FokI bound to dsDNA" /></td>
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    <tr>
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      <td><img style="width: 300px; height: 300px;"
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<td><img src="https://static.igem.org/mediawiki/2009/2/23/Freiburg09_huebsche_fokI_surface_render.jpg" name="Figure 2: FokI-Model surface rendering. DNA Strand is bound by two FOKI" width="300" height="300" /></td>
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alt=""
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</tr>
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src="https://static.igem.org/mediawiki/2009/9/99/Freiburg09_huebsche_fokI.jpg" /></td>
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      <td><img style="width: 300px; height: 300px;"
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alt=""
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<td><img src="https://static.igem.org/mediawiki/2009/6/6a/Freiburg09_fokI_3oligos.JPG" name="Schema 3: 2Oligos, both with active fokI, one cutting DNA, the other cutting a third oligo" width="300" height="300" /></td>
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src="https://static.igem.org/mediawiki/2009/2/23/Freiburg09_huebsche_fokI_surface_render.jpg" /></td>
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<td><img src="https://static.igem.org/mediawiki/2009/6/6e/Freiburg09_fokI_1inactive.JPG" name="Schema 4: 2Oligos, one with active fokI cutting DNA, the other inactive, just binding its own oligo" width="300" height="300" /></td>
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alt=""
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src="https://static.igem.org/mediawiki/2009/6/6a/Freiburg09_fokI_3oligos.JPG" /></td>
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<td><img src="https://static.igem.org/mediawiki/2009/8/8a/Freiburg09_fokI_1oligo.JPG" name="Schema 5: 1Oligo with active FokI cutting DNA, the other inactive, just binding its own oligo" width="300" height="300" /></td>
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      <td><img style="width: 300px; height: 300px;"
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alt=""
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<td><img src="https://static.igem.org/mediawiki/2009/4/4a/Freiburg09_fokI_pna.JPG" name="Schema 6: 2Oligo, with active fokI cutting DNA, attached on both FokI is a specific PNA. Oligos must be designed to bind this sequence. It is to be discussed whether there is a third oligo required for 3d stabilisation of dna" width="300" height="300" /></td>
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src="https://static.igem.org/mediawiki/2009/6/6e/Freiburg09_fokI_1inactive.JPG" /></td>
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alt=""
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src="https://static.igem.org/mediawiki/2009/8/8a/Freiburg09_fokI_1oligo.JPG" /></td>
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      <td><img style="width: 300px; height: 300px;"
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alt=""
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src="https://static.igem.org/mediawiki/2009/4/4a/Freiburg09_fokI_pna.JPG" /></td>
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Revision as of 14:17, 13 October 2009

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> FREiGEM

  April

24th April 12:30 - 18:00 o'clock Manuel

Literaturrecherche zu FokI.
Entstandene Ideen:
  • three oligo's: two for binding the monomers and one to create a dsDNA where FokI can bind.
  • two oligo's: one for binding one monomer, the second for binding the second monomer and to create dsDNA (one monomer has to be inactivated).
  • one oligo: both binding sites of the monomers on one oligo (also one monomer has to be inactivated).

The 196 C-terminale aminoacids of FokI were used from other groups (see reference 1). Primersequences are also provided in the publication.
Reference 1: Freiburg09_FokI_Zn-Finger_1996.pdf

29th april 21o'clock Rüdiger

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contact:  freigem09@googlemail.com

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