Template:Team:KULeuven/27 August 2009/VanillinReceptor

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  • Because of bad results yesterday the transformation of W was extra checked by performing a new PCR with different primers :

sample A : M13forward - virAforward sample B : M13forward - virAreverse sample C : virAforward - virAreverse sample D : M13reverse - virAforward sample E : M13reverse - virAreverse

Afterwards was gelelectrophoresis done and this showed virA was not present in the cell.

  • ligation of X and Y showed a pover result so new ligations were done for virB and virG
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