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From 2009.igem.org

Home The Project Motivation Solution Results & Conclusions References Materials & Methods Measurements Instruments Protocols Software Developed Parts Submitted Characterization The Team Notebook Week-book Freezer Management Biological Safety Pavia History University Gallery Sponsors Gallery Team Locations		Protocols
	LB medium DNA resuspension from iGEM 2009 plates Transformation X-Gal staining protocol for beta galactosidase (blue/white screening) Glycerol stocks Plasmid extraction BioBrick digestion with restriction enzymes Electrophoresis DNA gel extraction DNA precipitation with sodium acetate Ligation PCR Sensing ethanol concentration: our do-it-yourself kit

Glycerol stocks

(estimated time: 15 min + freezing time)

Mix 750 ul from the 5 ml sample of the incubated bacteria with 250 ul of 80% glycerol Leave at -20°C for one day Move to -80°C the day after

o/n or in exp phase bacterial culture 80% glycerol

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