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Protocols

• Lb medium
• Transformation
• X-Gal staining protocol for beta galactosidase (blue/white screening)
• Glycerol stock
• Plasmid extraction
• BioBrick digestion with restriction enzymes
• DNA gel extraction
• DNA precipitation with sodium acetate
• Ligation
• PCR

Gycerol stock (estimated time: 15 min + freezing time)

Materials needed:

• sample of overnight incubated bacteria
• 80% glycerol

• Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol
• Leave at -20°C for one day
• Move to -80°C the day after