The following Parts contain the JeT proximal promoter, and a core promoter as described. Core and proximal promoter are separated by a HindIII site. Parts can be applied for testing synthetic proximal promoters. The strength of promoters derived from these parts can be modified by core promoter swapping.
All contain the core promoter of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112] and were screened / measured in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100].
Name | Part type | Registry link
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Constitutive promoter; 0.4 REU | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203110 Part:BBa_K203110]
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Constitutive promoter; 1 REU | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203118 Part:BBa_K203118]
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Constitutive promoter; 2 REU | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203111 Part:BBa_K203111]
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Constitutive promoter; 2.5 REU | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203109 Part:BBa_K203109]
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NF-κB regulated promoter | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203119 Part:BBa_K203119]
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pPARγ regulated promoter 1 | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203114 Part:BBa_K203114]
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pPARγ regulated promoter 2 | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203115 Part:BBa_K203115]
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Protein domains for targeting to sub-cellular compartments.
Name | Part type | Registry link
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Plasma membrane targeting (GPI anchor) | Protein domain | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203000 Part:BBa_K203000]
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Plasma membrane targeting (myrpalm) | Protein domain | [http://partsregistry.org/Part:BBa_K203108 Part:BBa_K203108]
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ER membrane targeting (C-terminal) | Protein domain | [http://partsregistry.org/Part:BBa_K203101 Part:BBa_K203101]
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ER membrane targeting (N-terminal) | Protein domain | [http://partsregistry.org/Part:BBa_K203124 Part:BBa_K203124]
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