Team:British Columbia

From 2009.igem.org

The Bacterial Traffic Light

The goal of our project is to construct an Eschericia coli bacterial "traffic light", that responds to increasing concentration levels of a given input (in our case, arabinose) with green, yellow, and red fluorescent outputs. Our project is unique because it is an analog biosensor, capable of responding to a continuous variable signal, rather than a binary biosensor that only signals on/off.

Schematic black-box representation of the E. coli Biosensor that detects various concentration inputs and color outputs. The idea is discrete analog outputs based on a user-specified threshold for each range of concentration.

The Traffic Light is composed of three distinct subparts:

• The pBAD promoter family
• The lock and key riboregulation system
• The Jammer.

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