Uppsala-Sweden/15 October 2009

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Triparental mating

The transfer of PRKRYT to cynecosystis were started today awaiting to be continued tomorrow.


Evaluating transformations

The colonies that were picked yesterday were evaluated on a gel.

Colony pcr 091015.png

(From left)

1. Fermentas 1kb ladder

2-6. PRPRYT(AC) 1-5

7-11. PRKRYT(XAK) 1-5

12. Fermentas 1kb ladder


Colony pcr 091015 r2.png

(from left)

1. Fermentas 1kb ladder

2-6. PRPRAT(XAK) 1-5

7-11. PE1B3T(XAK) 1-5

12. Fermentas 1kb ladder


Inoculations of PRPRYT 2 & 4 and PRKRYT 4.


New colonies were pciked for the failing ones but with poor result again, as suspected. Only one colony for PRPRAT had the right length, and this one were inoculated as well.





Bioneer Biolegio Clontech Uppsala Genome Center

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