From 2009.igem.org

Arabinose sensor: the pBAD promoter

Overview

In order to make an analog biosensor, we need our traffic light to produce distinct, unique responses to a range of concentrations of an input. However, the Registry is lacking in variable strength inducible promoters. We designed two variants of the pBAD promoter, one weaker and one stronger than wild type, based on AraC binding experiments performed by Niland et al.

A diagram of the changed nucleotide sequences of arabinose-inducible promoters. Green nucleotides of the wildtype show all sequences that were mutated from the wild type to form strong and weak.

Above is a sequence alignment of our promoter variants with the wild type pBAD, truncated for space. For the full sequences, see our parts in the registry at (strong) and (weak).

We assembled each promoter with the RFP reporter part [http://partsregistry.org/wiki/index.php/Part:BBa_I13507 BBa_I13507] in order to test the relative activity of the mutated promoters

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