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CAROL
Isolation of Plasmids (pSB1AC3 and pSB1AK3)for Cloning
- Isolated plasmids (pSB1AK3 and pSB1AC3) from overnight cultures prepared by Thane.
- Protocol: Used GenElute Plasmid Miniprep Kit (Sigma-Aldrich), lot # 048k6062, used as according to the manufacturers instructions.
Plasmid | 260/280 | 260/230 | Concentration [ng/μL]
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psB1AC3 1 | 1.96 | 3.48 | 76.9
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psB1AC3 2 | 1.93 | 2.29 | 98.1
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psB1AC3 3 | 1.87 | 1.88 | 48.5
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psB1AC3 4 | 1.90 | 2.11 | 118.9
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psB1AK3 1 | 2.05 | 2.42 | 134.4
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psB1AK3 2 | 1.95 | 2.23 | 55.6
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psB1AK3 3 | 1.91 | 2.22 | 97.9
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psB1AK3 4 | 2.03 | 1.92 | 44.5
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- Set up restriction enzyme digest to ensure that biobrick sites are in the vector. The following are the reactions that were done:
Reaction 1: pSB1AC3 - cut with NotI
Reaction 2: pSB1AC3 - cut with EcoRI and SpeI
Reaction 3: pSB1AC3 - cut with XbaI and PstI
Reaction 4: pSB1AC3 - cut with XbaI and SpeI
Reaction 5: pSB1AK3 - cut with NotI
Reaction 6: pSB1AK3 - cut with EcorI and SpeI
Reaction 7: pSB1AK3 - cut with XbaI and PstI
Reaction 8: pSB1AK3 - cut with XbaI and SpeI
- Incubate reactions at 37oC
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CHINMOYEE
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EMILY
Purification of psB1AC3 & psB1AK3 vectors
- Protocol: Used GenElute Plasmid Miniprep Kit (Sigma-Aldrich), lot # 048k6062, used as according to the manufacturers instructions.
Plasmid | 260/280 | 260/230 | Concentration [ng/μL]
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psB1AC3 1 | 1.96 | 3.48 | 76.9
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psB1AC3 2 | 1.93 | 2.29 | 98.1
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psB1AC3 3 | 1.87 | 1.88 | 48.5
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psB1AC3 4 | 1.90 | 2.11 | 118.9
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psB1AK3 1 | 2.05 | 2.42 | 134.4
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psB1AK3 2 | 1.95 | 2.23 | 55.6
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psB1AK3 3 | 1.91 | 2.22 | 97.9
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psB1AK3 4 | 2.03 | 1.92 | 44.5
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FAHD
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
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MANDY
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PATRICK
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PRIMA
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STEFAN
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VICKI
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