Template:Team:KULeuven/29 July 2009/BlueLightReceptor
From 2009.igem.org
GFP ()
- miniprepped and nanodropped
- concentration: 85,6ng/μl
- 260/280: 1,87
- a restriction digest was performed to cut the plasmid with EcoRI and XbaI
- a mixture of 20μl was made:6μl DNA, 2μl bufferH, 1μl EcoRI and 1μl XbaI, 10μl AD
- the mixture was incubated for at least an hour at 37°C