From 2009.igem.org
Today Patrick arrived and began working on getting up to speed on the team's TTL model and creating the TTN model he will eventually be working on.
The model created was similar to that described previously except with the addition of a second tet operon (tetO2):
| Rxn #
| Reaction
| Forward Kinetic Constant
| Reverse Kinetic Constant
|
| 1/2 | RNAp + lacP + tetO1 + tetO2 ↔ RNAp:lacP | 1E+07 | 1
|
| 3 | RNA:lacP -> RNAp:lacP* | .01 |
|
| 4 | RNAp:lacP* -> lacP + tetO1 + tetO2 + RNAp:DNAgfp | 30 |
|
| 5 | RNAp:DNAgfp -> RNAp + gfp_mRNA | 30 |
|
| 6 | gfp_mRNA + rib -> rib:gfp_mRNA | 100000 |
|
| 7 | rib:gfp_mRNA -> rib:gfp_mRNA_1 + gfp_mRNA | 33 |
|
| 8 | rib:gfp_mRNA_1 -> rib + gfp | 33 |
|
| 9 | gfp_mRNA -> Ø | 1.16E-03 |
|
| 10 | gfp -> Ø | 3.21E-05 |
|
| 11\12 | tetR2 + aTc ↔ tetR2:aTc | 2E+09 | 4E-04
|
| 13\14 | tetR2:aTc + aTc ↔ tetR2:aTc2 | 1E+08 | 1E-03
|
| 15\16 | tetR2 + tetO1 ↔ tetR2:tetO1 | 1E+08 | 1E-03
|
| 17\18 | tetR2:aTc + tetO1 ↔ tetR2:tetO1:aTc | 1E+08 | 1
|
| 19\20 | tetR2:aTc2 + tetO1 ↔ tetR2:tetO1:aTc2 | 1E+08 | 1E+05
|
| 21\22 | tetR2:tetO1 + aTc ↔ tetR2:tetO1:aTc | 1E+08 | 1E-03
|
| 23\24 | tetR2:tetO1:aTc + aTc ↔ tetR2:tetO1:aTc2 | 1E+08 | 1E-03
|
| 25\26 | tetR2 + tetO2 ↔ tetR2:tetO2 | 1E+08 | 1E-03
|
| 27\28 | tetR2:aTc + tetO2 ↔ tetR2:tetO2:aTc | 1E+08 | 1
|
| 29\30 | tetR2:aTc2 + tetO2 ↔ tetR2:tetO2:aTc2 | 1E+08 | 1E+05
|
| 31\32 | tetR2:tetO2 + aTc ↔ tetR2:tetO2:aTc | 1E+08 | 1E-03
|
| 33\34 | tetR2:tetO2:aTc + aTc ↔ tetR2:tetO2:aTc2 | 1E+08 | 1E-03
|
| 35 | tetR2 -> Ø | 2.89E-04 |
|
| 36 | tetR2:aTc -> aTc | 2.89E-04 |
|
| 37 | tetR2:aTc2 -> 2 aTc | 2.89E-04 |
|
| 38\39 | tetR2 + nsDNA ↔ tetR2:nsDNA | 1000 | 3.2409
|
| 40\41 | tetR2:aTc + nsDNA ↔ tetR2:aTc:nsDNA | 1000 | 3.2409
|
| 42 | tetR2:aTc:nsDNA -> aTc + nsDNA | 1.93E-04 |
|
| 43 | tetR2:nsDNA -> nsDNA | 1.93E-04 |
|
| 44 | Ø -> tetR2 | 1E-11 |
|
| 45 | Ø -> aTc | 3.3E-04 |
|
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