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CAROL
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WIKI CODING HERE
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CHINMOYEE
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EMILY
Construction Begins!
Now that we have our gene of interest, LuxOD47E, Biobricked, we can start constuction to add the J13002 promoter and the B0015 terminator. We'll do two constrcutions in parallel and see if one of them works.
* Did a restriction digest cutting the insert with EcoRI and SpeI and cutting the recipient with EcoRI and XbaI restriction enzymes.
* Performed Antarctic Phosphotase Treatment (NEB) on the recipients, followed by ligation of the inserts and recipients.
*Transformed J13002-LuxOD47E into TOP10 cells and plated on AC and C plates in 25 uL and 50 uL, left plates for overnight growth.
*Did restreaks and made overnight cultures of colony2X LuxOD47E BBK.
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FAHD
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
1. Plasmid Isolation of fluorescent proteins
Isolation of fluorescent proteins were done in order to verify the presence and functionality of fluorescent proteins and promoters.
1. Testing of fluorescent proteins and the promoters
Isolated Flurorescent proteins were constructed behind of R0040 or J13002, depending on whether or not the fluorescent protein contained RBS, and transformed into TOP10 cells.
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MANDY
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PATRICK
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PRIMA
Marketing
Today I spoke to the President from BioAlberta and he generously offered to donate money to iGEM Calgary. BioAlberta was added to our Bronze list for sponsorship.
I also wrote up some emails to companies after researching the companies for the past few days. The rest of the marketing team edited the emails before I sent them off. I also call 8 other companies and 5 companies were interested in looking at our sponsorship package. I sent off the package and updated the iGEM marketing excel spreadsheet. I left a voicemail to the 3 companies and I'll follow up with them tomorrow or later this week. I got the contact information of the Marketing Lead for one of those 3 companies. My plan is to call the other 2 companies tomorrow to first: get a hold of someone in-charge of sponsorship and second: speak to them about our project, potential applications and how it may benefit their company.
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STEFAN
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VICKI
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