|
|
CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
|
|
|
CHINMOYEE
Math Model summary made
Today I went to the modelling meeting in ICT building . Then I decided to make a summary about math modelling so that on Friday both the modelling teams could compare the two system . This will ensure uniformity in parameter values , reaction equations used etc. Also a good written record will be present.
|
|
|
EMILY
J13002-LuxOD47E-B0015 Construction Take Two
- Today I ran a colony PCR of my J13002-LuxOD47E-B0015 colonies to verify the presence of the B0015 terminator in my construct. BBK-CP forward and reverse primers were used with p Taq and cycling conditions were as follows: 94°C for 6 min, 36x(94°C for 30 s, 55°C for 45s, 72°C for 90s), 72°Cfor 10 min, held at 4°C. PCR products were visualized on a 1% agarose gel run at 120 V with J13002-LuxOD47E and LuxOD47E as size controls. Gel is pictures below. Lanes 1-4 are trial one where the B0015 terminator was treated as the insert. Lanes 5-8 are trial 2 where the J13002-LuxOD47E construct was treated as the insert. Lane 9 is J13002-LuxOD47E and Lane 10 is LuxOD47E. Lane 11 is blank and Lane 12 is the negative control.
- Because all bands in the first 9 lanes appear to be the same size as the band in lane 9 with J13002-LuxOD47E, we can conclude that the B0015 terminator was not successfully cloned in and we will have to go back to contruction again.
- Performed a restriction digest with EcoRI, XbaI and SpeI of B0015 and the J13002-LuxOD47E construct, digesting the insert with EcoRI and SpeI and digesting the recipient with EcoRI and XbaI. Left the digestioin in the 37C waterbath for 2 hours, transformed into TOP10 cells on both AC and C plates and left for overnight growth.
|
|
|
FAHD
Marketing for july 9th 2009
1) I contacted a number of travel agencies(including Travel Cuts) and the cheapest i got was thru travel cuts and that was 595 CDN dollars(includs taxes and this price is with student discounts). However, Prima got a really good deal a few weeks ago( 412 CDN dollars) and i think(suggestion) that we should book these flights before somebody else grabs the booking. I am still waiting for our team members to respond me back with regards to the HOTELS e-mail i had sent out on WEDNESDAY abt their prefernece. SO far only Patrick, Mandy, Stefan and Prima have told me abt their preference.
2) I did a research/invesyigation on the following research pharmaceutical companies:
a-Ambion Inc.
b-Charles River Labs
c-Critical Outcome Technologies
d- E-Z-EM Canada Inc.
e- Genome Canada
f- i3 Canada Inc.
g- Ikaria Canada Inc.
h- Inimex Pharmaceuticals
i- Arc Pharma Canada Inc.
j- Dalto Pharma Services
k- Medicago Inc.
l- Neuroimage Inc.
m- Synbiosis
I e-mailed only 4 of the companies above because i want to spend as much time as i can on a company. I also have to confirm them with my team before sending them out so it takes time but i hope they respond back to us in a positive manner.
|
|
|
IMAN
Descriptive Title of What You're Doing
|
|
|
JAMIE
Descriptive Title of What You're Doing
|
|
|
JEREMY
Descriptive Title of What You're Doing
|
|
|
KATIE
Continuation of July 8th
I was able to complete scripts for three construction sites for the restriction digest activity that I planned out yesterday. The three construction sites will be used for creating a circuit from four parts: Promoter, RBS, terminator as well as a gene of the user’s choice. Currently it will only function for a general activity so I will have to modify it for specific missions. The construction sites consist of:
1. Single construction site for ligating two parts of the circuit together. These two parts must have been cut with the proper restriction enzymes to place one before the other ie. Promoter cannot be placed behind RBS.
2. Multiple construction site that works the same as the first but with two parts already together that must be connected to another part.
3. Multiple construction site with three pieces together already. There is also an option to ligate Promoter/RBS with Gene/Terminator, which can actually be considered a shortcut as long as users understand what they are doing.
One flaw in the activity is that you are only able to cut each piece once in the donor and recipient tubes, but I thought it would be unnecessarily tedious to cut at each step of construction once they have already succeeded in doing it once, but this can always be changed although I do not plan to in the upcoming week. It would involve possibly doubling the amount of comparisons within the donor and recipient tubes avatars may use to determine what they wish to cut. I also began to modify the recipient and donor tube scripts in order for them to cooperate with the three construction sites so they are no longer fully functional as I continue to change them. I have determined the names of the products an avatar may obtain from performing the activity so now I will have to create them and request they be given to inventory, which I hope to complete by next week and will be starting tomorrow.
I will most likely finish the restriction digest notecard in parallel with its corresponding activity and I will be sending these notecards to Mandy once again to look over and examine their validity, which I plan to have done by Monday (July 13). I was also able to construct the basic plates for streaking for the bacterial transformation activity.
I was also able to complete the script for the sequencing station as per Mandy’s request and it will now give any avatar who drops ‘Amplified Colison E2’ into the sequencing object a notecard, which as of now does not contain any useful information. I will be adding various other amplified DNA products as objects to give back sequencing for on Friday, which may continue into next week.
|
|
|
KEVIN
The results of the restreaks of J13002 and GFP/RFP
The following is an image of the restreak:
From it, one can clearly see that RFP is glowing much brighter than the negative control, and that GFP is, although less obvious, also glowing.
No other lab work was performed on this day.
|
|
|
MANDY
Descriptive Title of What You're Doing
|
|
|
PATRICK
Descriptive Title of What You're Doing
|
|
|
PRIMA
Descriptive Title of What You're Doing
|
|
|
STEFAN
Descriptive Title of What You're Doing
|
|
|
VICKI
Descriptive Title of What You're Doing
|
"
