Team:Calgary/14 July 2009

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University of Calgary

UNIVERSITY OF CALGARY



THIS MONTH

July
MTWTFSS
    [http://2009.igem.org/Team:Calgary/1_July_2009 1] [http://2009.igem.org/Team:Calgary/2_July_2009 2] [http://2009.igem.org/Team:Calgary/3_July_2009 3] [http://2009.igem.org/Team:Calgary/4_July_2009 4] [http://2009.igem.org/Team:Calgary/5_July_2009 5]
[http://2009.igem.org/Team:Calgary/6_July_2009 6] [http://2009.igem.org/Team:Calgary/7_July_2009 7] [http://2009.igem.org/Team:Calgary/8_July_2009 8] [http://2009.igem.org/Team:Calgary/9_July_2009 9] [http://2009.igem.org/Team:Calgary/10_July_2009 10] [http://2009.igem.org/Team:Calgary/11_July_2009 11] [http://2009.igem.org/Team:Calgary/12_July_2009 12]
[http://2009.igem.org/Team:Calgary/13_July_2009 13] [http://2009.igem.org/Team:Calgary/14_July_2009 14] [http://2009.igem.org/Team:Calgary/15_July_2009 15] [http://2009.igem.org/Team:Calgary/16_July_2009 16] [http://2009.igem.org/Team:Calgary/17_July_2009 17] [http://2009.igem.org/Team:Calgary/18_July_2009 18] [http://2009.igem.org/Team:Calgary/19_July_2009 19]
[http://2009.igem.org/Team:Calgary/20_July_2009 20] [http://2009.igem.org/Team:Calgary/21_July_2009 21] [http://2009.igem.org/Team:Calgary/22_July_2009 22] [http://2009.igem.org/Team:Calgary/23_July_2009 23] [http://2009.igem.org/Team:Calgary/24_July_2009 24] [http://2009.igem.org/Team:Calgary/25_July_2009 25] [http://2009.igem.org/Team:Calgary/26_July_2009 26]
[http://2009.igem.org/Team:Calgary/27_July_2009 27] [http://2009.igem.org/Team:Calgary/28_July_2009 28] [http://2009.igem.org/Team:Calgary/29_July_2009 29] [http://2009.igem.org/Team:Calgary/30_July_2009 30] [http://2009.igem.org/Team:Calgary/31_July_2009 31]


NOTEBOOK PAGE INDEX



CALENDAR

May
MTWTFSS
        [http://2009.igem.org/Team:Calgary/1_May_2009 1] [http://2009.igem.org/Team:Calgary/2_May_2009 2] [http://2009.igem.org/Team:Calgary/3_May_2009 3]
[http://2009.igem.org/Team:Calgary/4_May_2009 4] [http://2009.igem.org/Team:Calgary/5_May_2009 5] [http://2009.igem.org/Team:Calgary/6_May_2009 6] [http://2009.igem.org/Team:Calgary/7_May_2009 7] [http://2009.igem.org/Team:Calgary/8_May_2009 8] [http://2009.igem.org/Team:Calgary/9_May_2009 9] [http://2009.igem.org/Team:Calgary/10_May_2009 10]
[http://2009.igem.org/Team:Calgary/11_May_2009 11] [http://2009.igem.org/Team:Calgary/12_May_2009 12] [http://2009.igem.org/Team:Calgary/13_May_2009 13] [http://2009.igem.org/Team:Calgary/14_May_2009 14] [http://2009.igem.org/Team:Calgary/15_May_2009 15] [http://2009.igem.org/Team:Calgary/16_May_2009 16] [http://2009.igem.org/Team:Calgary/17_May_2009 17]
[http://2009.igem.org/Team:Calgary/18_May_2009 18] [http://2009.igem.org/Team:Calgary/19_May_2009 19] [http://2009.igem.org/Team:Calgary/20_May_2009 20] [http://2009.igem.org/Team:Calgary/21_May_2009 21] [http://2009.igem.org/Team:Calgary/22_May_2009 22] [http://2009.igem.org/Team:Calgary/23_May_2009 23] [http://2009.igem.org/Team:Calgary/24_May_2009 24]
[http://2009.igem.org/Team:Calgary/25_May_2009 25] [http://2009.igem.org/Team:Calgary/26_May_2009 26] [http://2009.igem.org/Team:Calgary/27_May_2009 27] [http://2009.igem.org/Team:Calgary/28_May_2009 28] [http://2009.igem.org/Team:Calgary/29_May_2009 29] [http://2009.igem.org/Team:Calgary/30_May_2009 30] [http://2009.igem.org/Team:Calgary/31_May_2009 31]


June
MTWTFSS
[http://2009.igem.org/Team:Calgary/1_June_2009 1] [http://2009.igem.org/Team:Calgary/2_June_2009 2] [http://2009.igem.org/Team:Calgary/3_June_2009 3] [http://2009.igem.org/Team:Calgary/4_June_2009 4] [http://2009.igem.org/Team:Calgary/5_June_2009 5] [http://2009.igem.org/Team:Calgary/6_June_2009 6] [http://2009.igem.org/Team:Calgary/7_June_2009 7]
[http://2009.igem.org/Team:Calgary/8_June_2009 8] [http://2009.igem.org/Team:Calgary/9_June_2009 9] [http://2009.igem.org/Team:Calgary/10_June_2009 10] [http://2009.igem.org/Team:Calgary/11_June_2009 11] [http://2009.igem.org/Team:Calgary/12_June_2009 12] [http://2009.igem.org/Team:Calgary/13_June_2009 13] [http://2009.igem.org/Team:Calgary/14_June_2009 14]
[http://2009.igem.org/Team:Calgary/15_June_2009 15] [http://2009.igem.org/Team:Calgary/16_June_2009 16] [http://2009.igem.org/Team:Calgary/17_June_2009 17] [http://2009.igem.org/Team:Calgary/18_June_2009 18] [http://2009.igem.org/Team:Calgary/19_June_2009 19] [http://2009.igem.org/Team:Calgary/20_June_2009 20] [http://2009.igem.org/Team:Calgary/21_June_2009 21]
[http://2009.igem.org/Team:Calgary/22_June_2009 22] [http://2009.igem.org/Team:Calgary/23_June_2009 23] [http://2009.igem.org/Team:Calgary/24_June_2009 24] [http://2009.igem.org/Team:Calgary/25_June_2009 25] [http://2009.igem.org/Team:Calgary/26_June_2009 26] [http://2009.igem.org/Team:Calgary/27_June_2009 27] [http://2009.igem.org/Team:Calgary/28_June_2009 28]
[http://2009.igem.org/Team:Calgary/29_June_2009 29] [http://2009.igem.org/Team:Calgary/30_June_2009 30]


July
MTWTFSS
    [http://2009.igem.org/Team:Calgary/1_July_2009 1] [http://2009.igem.org/Team:Calgary/2_July_2009 2] [http://2009.igem.org/Team:Calgary/3_July_2009 3] [http://2009.igem.org/Team:Calgary/4_July_2009 4] [http://2009.igem.org/Team:Calgary/5_July_2009 5]
[http://2009.igem.org/Team:Calgary/6_July_2009 6] [http://2009.igem.org/Team:Calgary/7_July_2009 7] [http://2009.igem.org/Team:Calgary/8_July_2009 8] [http://2009.igem.org/Team:Calgary/9_July_2009 9] [http://2009.igem.org/Team:Calgary/10_July_2009 10] [http://2009.igem.org/Team:Calgary/11_July_2009 11] [http://2009.igem.org/Team:Calgary/12_July_2009 12]
[http://2009.igem.org/Team:Calgary/13_July_2009 13] [http://2009.igem.org/Team:Calgary/14_July_2009 14] [http://2009.igem.org/Team:Calgary/15_July_2009 15] [http://2009.igem.org/Team:Calgary/16_July_2009 16] [http://2009.igem.org/Team:Calgary/17_July_2009 17] [http://2009.igem.org/Team:Calgary/18_July_2009 18] [http://2009.igem.org/Team:Calgary/19_July_2009 19]
[http://2009.igem.org/Team:Calgary/20_July_2009 20] [http://2009.igem.org/Team:Calgary/21_July_2009 21] [http://2009.igem.org/Team:Calgary/22_July_2009 22] [http://2009.igem.org/Team:Calgary/23_July_2009 23] [http://2009.igem.org/Team:Calgary/24_July_2009 24] [http://2009.igem.org/Team:Calgary/25_July_2009 25] [http://2009.igem.org/Team:Calgary/26_July_2009 26]
[http://2009.igem.org/Team:Calgary/27_July_2009 27] [http://2009.igem.org/Team:Calgary/28_July_2009 28] [http://2009.igem.org/Team:Calgary/29_July_2009 29] [http://2009.igem.org/Team:Calgary/30_July_2009 30] [http://2009.igem.org/Team:Calgary/31_July_2009 31]


August
MTWTFSS
          [http://2009.igem.org/Team:Calgary/1_August_2009 1] [http://2009.igem.org/Team:Calgary/2_August_2009 2]
[http://2009.igem.org/Team:Calgary/3_August_2009 3] [http://2009.igem.org/Team:Calgary/4_August_2009 4] [http://2009.igem.org/Team:Calgary/5_August_2009 5] [http://2009.igem.org/Team:Calgary/6_August_2009 6] [http://2009.igem.org/Team:Calgary/7_August_2009 7] [http://2009.igem.org/Team:Calgary/8_August_2009 8] [http://2009.igem.org/Team:Calgary/9_August_2009 9]
[http://2009.igem.org/Team:Calgary/10_August_2009 10] [http://2009.igem.org/Team:Calgary/11_August_2009 11] [http://2009.igem.org/Team:Calgary/12_August_2009 12] [http://2009.igem.org/Team:Calgary/13_August_2009 13] [http://2009.igem.org/Team:Calgary/14_August_2009 14] [http://2009.igem.org/Team:Calgary/15_August_2009 15] [http://2009.igem.org/Team:Calgary/16_August_2009 16]
[http://2009.igem.org/Team:Calgary/17_August_2009 17] [http://2009.igem.org/Team:Calgary/18_August_2009 18] [http://2009.igem.org/Team:Calgary/19_August_2009 19] [http://2009.igem.org/Team:Calgary/20_August_2009 20] [http://2009.igem.org/Team:Calgary/21_August_2009 21] [http://2009.igem.org/Team:Calgary/22_August_2009 22] [http://2009.igem.org/Team:Calgary/23_August_2009 23]
[http://2009.igem.org/Team:Calgary/24_August_2009 24] [http://2009.igem.org/Team:Calgary/25_August_2009 25] [http://2009.igem.org/Team:Calgary/26_August_2009 26] [http://2009.igem.org/Team:Calgary/27_August_2009 27] [http://2009.igem.org/Team:Calgary/28_August_2009 28] [http://2009.igem.org/Team:Calgary/29_August_2009 29] [http://2009.igem.org/Team:Calgary/30_August_2009 30]
[http://2009.igem.org/Team:Calgary/31_August_2009 31]


September
MTWTFSS
  [http://2009.igem.org/Team:Calgary/1_September_2009 1] [http://2009.igem.org/Team:Calgary/2_September_2009 2] [http://2009.igem.org/Team:Calgary/3_September_2009 3] [http://2009.igem.org/Team:Calgary/4_September_2009 4] [http://2009.igem.org/Team:Calgary/5_September_2009 5] [http://2009.igem.org/Team:Calgary/6_September_2009 6]
[http://2009.igem.org/Team:Calgary/7_September_2009 7] [http://2009.igem.org/Team:Calgary/8_September_2009 8] [http://2009.igem.org/Team:Calgary/9_September_2009 9] [http://2009.igem.org/Team:Calgary/10_September_2009 10] [http://2009.igem.org/Team:Calgary/11_September_2009 11] [http://2009.igem.org/Team:Calgary/12_September_2009 12] [http://2009.igem.org/Team:Calgary/13_September_2009 13]
[http://2009.igem.org/Team:Calgary/14_September_2009 14] [http://2009.igem.org/Team:Calgary/15_September_2009 15] [http://2009.igem.org/Team:Calgary/16_September_2009 16] [http://2009.igem.org/Team:Calgary/17_September_2009 17] [http://2009.igem.org/Team:Calgary/18_September_2009 18] [http://2009.igem.org/Team:Calgary/19_September_2009 19] [http://2009.igem.org/Team:Calgary/20_September_2009 20]
[http://2009.igem.org/Team:Calgary/21_September_2009 21] [http://2009.igem.org/Team:Calgary/22_September_2009 22] [http://2009.igem.org/Team:Calgary/23_September_2009 23] [http://2009.igem.org/Team:Calgary/24_September_2009 24] [http://2009.igem.org/Team:Calgary/25_September_2009 25] [http://2009.igem.org/Team:Calgary/26_September_2009 26] [http://2009.igem.org/Team:Calgary/27_September_2009 27]
[http://2009.igem.org/Team:Calgary/28_September_2009 28] [http://2009.igem.org/Team:Calgary/29_September_2009 29] [http://2009.igem.org/Team:Calgary/30_September_2009 30]


October
MTWTFSS
      [http://2009.igem.org/Team:Calgary/1_October_2009 1] [http://2009.igem.org/Team:Calgary/2_October_2009 2] [http://2009.igem.org/Team:Calgary/3_October_2009 3] [http://2009.igem.org/Team:Calgary/4_October_2009 4]
[http://2009.igem.org/Team:Calgary/5_October_2009 5] [http://2009.igem.org/Team:Calgary/6_October_2009 6] [http://2009.igem.org/Team:Calgary/7_October_2009 7] [http://2009.igem.org/Team:Calgary/8_October_2009 8] [http://2009.igem.org/Team:Calgary/9_October_2009 9] [http://2009.igem.org/Team:Calgary/10_October_2009 10] [http://2009.igem.org/Team:Calgary/11_October_2009 11]
[http://2009.igem.org/Team:Calgary/12_October_2009 12] [http://2009.igem.org/Team:Calgary/13_October_2009 13] [http://2009.igem.org/Team:Calgary/14_October_2009 14] [http://2009.igem.org/Team:Calgary/15_October_2009 15] [http://2009.igem.org/Team:Calgary/16_October_2009 16] [http://2009.igem.org/Team:Calgary/17_October_2009 17] [http://2009.igem.org/Team:Calgary/18_October_2009 18]
[http://2009.igem.org/wiki/index.php?title=Team:Calgary/19_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/20_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/21_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/22_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/23_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/24_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/25_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=Team:Calgary/26_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/27_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/28_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/29_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/30_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/31_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 31]



JULY 14, 2009


CAROL

Descriptive Title of What You’re Doing

WIKI CODING HERE

CHINMOYEE

E-mailed Gauntlet about article

I looked at papers Potential : Kinetic Analysis of Multi site phosphorylation using Analytic Solutions to Michaelis-Menten Equations Ligand induced Asymmetry in HIstidine sensor kinase complex regulates quorum sensing


Dead end : Purification and characterization of a luxO promotor....



Non Paper related stuff : found a Systems biology toolbox for Matlab ( free) E-mailed gauntlet about writing a Frosh supplement article



EMILY

Construction of J13002-LuxOD47E-B0015, Glycerol Stocks and Transformation of J13002-LuxOD47E into KT1144 Cells

  • Today I did another contruction digest with J13002-LuxOD7E and B0015. I then transformed into TOP10 cells and plated on AC plates. I also made glycerol stocks of LuxOD47E BBK and of J13002-LuxOD47E.
  • The retreaks of the J13002-LuxOD47E contructs in KT1144 cells worked, however when tested under UV light, they still did not glow. I transformed a different colony of J13002-LuxOD47E into KT1144 cells again and we'll see if it gives any better results.
  • Today I also helped Mandy out with some stuff for the Wiki. I labelled, tagged and resized some photos that we're putting in our gallery and I wrote up a section for our Overall Project page that explains what the iGEM competition is and how our team is divided.


FAHD

Marketing for July 14th 2009

Today, I concentrated my energies at marketing our iGEM project. Here is what I did today:

1) Attended the meeting in the morning

2) Contacted VWR. Will do a follow up call soon.

3) Called EMD Chemicals/Biosciences (Brands include Novagen, Novabiochem and Calbiochem), left a voicemail and i will get back to them tomorrow.

4) Called Critical Outcome Technologies, nobody was available so i left them a voicemail.

5) Called Life Technologies. Lefet them a voicemail

6) I called the owner of Neuroimage Inc., I have telephone meeting with him on friday 6 pm(ET).

7) I e-mailed Jen a thank you letter after consulting my beautiful marketing team.

8) I replied to Mrs.Kubik e-mail abt the update on the e-mails that we had sent. Conoco Phillips and Statoil said no i will send them a 'rejection thank you' e-mail.

9) I discussed the template and information for the July's newsletter.

10)I worked on the Air Canada Community investment application. They offer free airline tickets in their community investment programme.

11) I will go to walmart in the morning(8.30) for my visa photos cuz they messed up the photos last time. Then i will go to Scotia Bank to deposit my visa money hopefully by by 9.30/10.00.

12) I also called Genome Canada, I talked to the President's assistant: she told me to me to e-mail her the proposal so she will forward the e-mail to her boss.

13) I called Astellas Pharma however she is out of her office till next week.


IMAN

Descriptive Title of What You're Doing

WIKI CODING HERE


JAMIE

Descriptive Title of What You're Doing

WIKI CODING HERE


JEREMY

Descriptive Title of What You're Doing

WIKI CODING HERE


KATIE

Complete Version of Activities

This morning, I completed the restriction digest activity in its general form. Now, I have to rename materials so they are no longer general and add the notecards to be given to avatars to each object in the activity and set it so that you cannot read what is within the notecard, which will be compared to a master notecard within the sequencer at the end of the activity. This prevents anyone from just renaming objects in their inventory (“cheating”). I am in the process of modifying the notecard reader script for use within the sequencer and depending on how complicated it ends up being I may incorporate it into the rest of the restriction digest activity and check all notecards for every step if possible. My restriction digest notecard has also been completed to the best of my ability, which will be sent to Mandy this week.

I am now working on bacterial transformation, which I would like to be slightly different from PCR/Gel Electrophoresis and Restriction digest, which have functions different from one another as well (for example, order is important for one). So, I believe this activity will ask the user questions to assure they have some idea of what they are doing, which once again also serves the secondary purpose of preventing people from just renaming objects and being able to complete the activity.

I have created an object that has the ability to create the bacteria avatars by touching it. Problem: Mandy pointed out that the attach avatar permission only worked for me, which I determined was because I am the owner of the object when it is rezzed. So instead of rezzing the object I decided to give the object to people so that they will become the owner. This means they will now have to take it out of their inventory by attaching it to themselves from their inventory. So it is working, just not the way I would prefer it. There is also varying levels of brightness for the bacteria, which I am unsure of whether to keep or just have it glow when there is enough bacteria.

Tomorrow I will modify the PCR script so that the items for each activity are more specific to that single activity as well as scientifically accurate, including the names for each specific PCR. I will be completing bacterial transformation after I brainstorm questions.

I also have to go back to the water bath script and incorporate it into the activities as well because as of now it is just a stand-alone activity. All I will have to do is change the names of what is added and given to and from the water bath respectively and this also means modifying phosphatase treatment. I am also beginning to make more visual instructions for restriction digest. So far sections that are explained will flash as instructions for that specific section are given. I would like to expand on this later this week.


KEVIN

Colony PCR (cPCR) of Pqrr4+I13500

Unlike the R0040 promoter driving the expression of I13500, Pqrr4 promoter is not constitutive, so it cannot be tested visually. Since I do not have any overnight cultures to perform Restriction Digest as of now, the only method I can use today is cPCR. 3 colonies were chosen, and 2 different sets of primers were used: BBK CP F/R primers and Pqrr4 F and BBK CP R. The following is a picture of the gel:

2009.07.14.Qrr4 I13500.png


Only colony 3 seemeed to have worked. Pqrr4+I13500 is about 1013bp, and because biobrick primers anneal outside of the circuit (adding about 250 more base pairs), it seems to match the band in the lane of colony 3. Since I only tried 3 colonies, tomorrow, I will pick more and perform another cPCR.


MANDY

Descriptive Title of What You're Doing

WIKI CODING HERE


PATRICK

Descriptive Title of What You're Doing

WIKI CODING HERE


PRIMA

Cold Calls & Transformation of Cl lambda into xl gold

For marketing: The marketing team sat down and decided that we should have our third bake on Monday, July 27th and the fourth one on Monday, August 16th. These dates are tentative. Fahd and I have worked on the July newsletter template: cedarlane is our sponsor of the month. Our goal is to get it done by Friday so we can start sending it out next week. For companies: Company 1 – got an email back saying they’re considering our sponsorship packgage  follow up with them on Monday, July 20th Company 2– couldn’t get a hold of them  call back tomorrow Company 3 – considering our package, needs some time  follow up on Monday July 20th Company 4 – sent package yesterday, will follow up tomorrow to see if they got my email Company 5 – (this was one of the older companies who said no…but I got a hold of someone new) –sent sponsorship package yesterday – will follow up tomorrow to see if they received it Company 6 –package sent to the executive committee, now considering  follow up July 20th Company 7 – sent package  follow up tomorrow to see if they received it Company 8- got an email today …refused to help us. I Sent them a thank you letter anyways and asked for contacts Company 9 - sent pkg, have CEO’s number, called before couldn’t get a hold of him, will call again tomorrow – I wanna set up a meeting with him cuz I think I know where their main office is located. Tomorrow – call the 4 companies from Mrs. Kubik’s company contacts. They were Suncore, Syncrude and 2 more whose names I don’t remember and Fahd took the list home. I sent them our package 2 weeks ago, tomorrow I’ll follow up to see if they had considered sponsoring us.

Lab: I helped Jeremy set up sequencing for LuxPQ-B-R-LuxOU-B in AK for colony 12. We used colony 12 because the restriction digest from yesterday worked perfectly for that colony (correct size and no random fragments) (please refer to attachment). Since last time we only got one colony from transforming Cl lambda couple days ago and the restriction digest we ran yesterday showed multiple bands, we couldn’t judge the size of Cl lambda properly. Therefore, today we decided to transform Cl lambda again into competent cells. Since the inverter plasmid is resistant to Kan, we made more Kan agar plates and then transformed the Cl lambda and let them grow over night. We also made overnight cultures of the Cl lamda. We’ll isolate the plasmids tomorrow. Then in the afternoon, if we see single colonies on our overnight plates, we ‘ll do restriction digest again to confirm that the Cl lambda is actually there in our plasmids.



STEFAN

Adding life to the Synthetic Kingdom

Today was spent on working on the synthetic kingdom. Difficulty was encountered because moving the giant eukaryotic cell was harder than it should've been. The organelles kept wanting to move to their original places because of the scripts involved so I had to stop all of them and delete them. I will reintroduce them once I have found a suitable place for the cell. We had 3 new "births" today, which increased the population of the bacteria! Squid were added as well as jellyfish and coral. I feel that this is important because of the relation of each to quorum sensing, GFP and RFP respectively so we can show that some genes from animals can be added to E.coli (pretend that is italicized). Also it looks cool. I am attempting to also pretty up the place so it will be more vibrant and full of life. This is going to be carried out by designing underwater plants and adding rocks as well for a more natural look. This will be carried out tomorrow.

Upon my domain reaching satisfactory levels, I did my blog video in one AMAZING take. Unfortunately it didn't save properly so now I have to do it at home tonight.


VICKI

Descriptive Title of What You're Doing

WIKI CODING HERE