"
August/8 August 2009
From 2009.igem.org
Today we carried out the following procedures (in rough chronological order):
1. Checked the cell cultures transformed and plated out yesterday (8/7) for colony formation and made an approximate count of the number of colonies.
(plate number)-(location on plate) (no. of colonies)
2-12H none
1-12C none
1-12A none
2-24G none
2-18F none
1-6O ~10
1-10K ~10
1-18L ~10
2. Conducted a 'miniprep' to harvest and check concentration of plasmids from 10 sample. (incubated since yesterday 8/7~) Result of Nanodrop concentration check:
(sample No.) (concentration) [260/280] [260/230]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 B0015](1-23L) 24.9 ng/uL 1.77 1.77
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K118002 K118002](2-16F) 36.0 ng/uL 1.77 1.99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I0462 I0462](1-8O) 19.3 ng/uL 1.65 1.59
[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034](1-2M) 18.4 ng/uL 1.64 2.01
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I742158 I742158](1-19B) 40.9 ng/uL 1.56 0.83
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K118003 K118003](2-16H) 27.7 ng/uL 1.68 1.38
[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 C0076](1-14D) 9.3 ng/uL 1.66 2.31
[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0179 C0179](2-8M) 9.9 ng/uL 1.65 1.90
[http://partsregistry.org/wiki/index.php?title=Part:BBa_S03878 S03878](2-16M) 24.6 ng/uL 1.72 1.24
[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 C0077](1-14F) 2.5 ng/uL 1.11 0.25 failure?
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I1466 I1466](1-23J) failure
3. gel electrophoresis
| uL | |
| EcoRI | 1 |
| SpeI | 1 |
| 10*Buffer No.2 | 2 |
| DNA (EpsE) | 5 |
| total | 20 |
↓
37℃,22hr
↓
failure (SYBR Safe : 30min???)
