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CAROL
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WIKI CODING HERE
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CHINMOYEE
CLASS
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EMILY
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FAHD
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IMAN
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JAMIE
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JEREMY
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KATIE
Back to the Lab after Completing Initial Version of Quorum Sensing
Quorum sensing bacteria are complete and I made bacteria with RFP and placed them beside other bacteria for display. I will probably return to work on this later, but it is now more important to finish the lab. I learned that the order in which you add materials during restriction digest is important so using the function (initially from cleaning script) created for storing the names of items in an inventory will be important to make sure that each time something is added, it is the correct item. I believe for the rest of the activity I may be able to use a modified version of my find item script.
However, I have yet to determine a new way to move things to the water bath, heating block etc. in a creative way and still keep avatars involved.
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KEVIN
1. Plasmid Isolation of R0040, B0015, and J13002
R0040 (promoter), B0015 (terminator), and J13002 (RBS+Promoter) were isolated in order to verify the presence of them with Restriction digest. These parts are needed for general construction of our circuits.
2. Restriction digest of R0040, B0015, and J13002
To verify if B0015, R0040, and J13002 are present within the plasmid/cell, restriction digest was performed using NotI enzyme.
The band in R0040 C2 #1 is the only one that did not match the expected size of each. Only one colony is needed to move on.
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MANDY
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PATRICK
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PRIMA
June Newsletter
In the moring, I was at the Biosafety training on main campus. (9:00am- 12:00 noon).
Later that afternoon, Jamie, Fahd and I continued to work on the June newsletter. It was our goal to have it done by today. We have sent it to Sonja and Thane to double check it before we send it out. We are hoping to begin sending it out starting next week.
I made 20 colorful bake sale posters to put around the health science building. Fahd and I also made the bake sale event on facebook and sent it to all of our friends and encouraged them to bring their friends. We also emailed the Health Science Communications coordinator to send an email out to all Health Science students to advertise our bake sale. Her generous efforts are greatly appreciated by the team.
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STEFAN
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VICKI
NotI restriction digest of isolated LuxOD47A on psB1AC3 constructs.
Purpose: Even though the colony PCR from 2 days ago had negative control contamination, we’re going to run a NotI restriction digest anyway. If we can excise a gene of the appropriate size, it is likely that the sample in question actually contains LuxOD47A on psB1AC3, as opposed to mere master mix contamination.
Protocol: NotI restriction enzymes were used with REact I buffer, in accordance with the restriction digest procedure outlined on the protocol page.
Results: As shown below, the 4th set of digested genes displayed the set of bands that we expected: a band around 1400 bp (accounting for LuxOD47A) and a band around 3000 bp (accounting for psB1AC3). We will use this sample in our later constructions.
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