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CAROL
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WIKI CODING HERE
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CHINMOYEE
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EMILY
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FAHD
Team Presentations
Today we had our team presentations. I presented on behalf of the Human Practices Aspect of our project.
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
Transformation of fluorescent proteins
I transformed E0040 (GFP), E0032 (YFP), E0022 (CFP), I13500 (GFP+RBS), and I13502 (RFP+RBS) into TOP10 cells for testing of R0040 and... for drawing bacterial pictures! Orange FP and Blue FP were not available for distribution, and purple FP was not added to the iGEM registry.
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MANDY
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PATRICK
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PRIMA
Isolate Plasmids
-Shadowed Jeremy today.
Purpose: isolate plasmids from overnight cultures containing Top 10 competent cells with Lux PQ in psB1AC3
Protocol: Geneflute Plasmid mini prep kit
After mini-prep we nano-dropped to determine the plasmid concentratoin at 260 wavelength
Plasmid | 260/280 | 260/230 | Concentration [ng/μL]
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LuxPQ Top 10 C24C11 | 1.82 | 2.23 | 160.2
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LuxPQ Top 10 C24C12 | 1.89 | 2.34 | 112.8
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LuxPQ psB1AC3 C11 | 1.89 | 2.17 | 89.4
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LuxPQ psB1AC3 C6 | 1.88 | 2.11 | 82.5
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LuxPQ psB1AC3 C7 | 1.88 | 2.09 | 79.0
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For sequencing:
C7 = send 8.34 microL
Sequencing Table
Reaction # | Name' | size | Primer | Tm
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1 | LuxPQ-BBK-C6-F | 6680 | BBK-CP-F | 55
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2 | LuxPQ-BBK-C6-R | 6680 | BBK-CP-R | 55
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3 | LuxPQ-BBK-C7-F | 6680 | BBK-CP-F | 55
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4 | LuxPQ-BBK-C7-R | 6680 | BBK-CP-R | 55
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STEFAN
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VICKI
Canada day make-up
No work done today, owing to the long weekend.
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