Team:Stanford/Project Homeostasis/sequences

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Home Team Project Parts Notebook Archives


Project
Research Proposal
Systems Overview
Cloning Plan
Sequences & Primers
Anti-Inflammation
Device Overview
Parts Design
Challenges
Results
Anti-Immunosuppression
Device Overview
Parts Design
Challenges
Results
Protocols
Modeling
Overview
Notebook
Results
Future Work
Archived Work



Contents

Device 1

RBS – SoxR gene – Terminator – Spacer – SoxS Promoter click [http://openwetware.org/wiki/IGEM:Stanford/2009/Project_Homeostasis/sequences/SoxRS here]


SoxR gene click [http://openwetware.org/wiki/IGEM:Stanford/2009/Project_Homeostasis/sequences/SoxR here]


SoxS Promoter click [http://openwetware.org/wiki/IGEM:Stanford/2009/Project_Homeostasis/sequences/SoxSPromoter here]


RBS - Blh gene click [http://openwetware.org/wiki/IGEM:Stanford/2009/Project_Homeostasis/sequences/Blh here]


RBS - RALDH II gene - Terminator click [http://openwetware.org/wiki/IGEM:Stanford/2009/Project_Homeostasis/sequences/RaldhII here]


hIL-6 hlyAs click [http://openwetware.org/wiki/IGEM:Stanford/2009/Project_Homeostasis/sequences/Il6HlyAs here]


Primer Design Guidelines


Resources

  • National Center for Biotechnology Information (NCBI)- Get Your Sequences

[http://www.ncbi.nlm.nih.gov/ Home Page]

  • Parts Registry of BioBricks- Help with Prefix and Suffix

[http://partsregistry.org/Help:BioBrick_Prefix_and_Suffix Prefix and Suffix Help]

  • IDT Integrated DNA Technologies- Check for %GC (40-60) and Melting Temp. (52-68C)

[http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/ Analyze Your Sequence]

  • NUPACK Nucleic Acid Package- check both primers for hairpins and trouble spots (No Red!!)

[http://nupack.org/partition/new Analzye both Primers]

  • ApE- A Plasmid Editor- Useful tool for checking sequences and finding reverse complements

[http://www.biology.utah.edu/jorgensen/wayned/ape/ Download here]

  • Ginkgo BioWorks- creates primers w/ BioBrick additions

[http://ginkgobioworks.com/cgi/primer.cgi Design Primers Here]


Archived Documents and Work

  • Past Primers and Sequences
    • [http://openwetware.org/wiki/IGEM:Stanford/2009/Project_Homeostasis/Primer/Primersdoc Finalized Original Primers]
    • [http://openwetware.org/wiki/IGEM:Stanford/2009/Project_Homeostasis/Primer Original Primers]
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