"

 

From 2009.igem.org

Home The Project Motivation Solution Results & Conclusions References Materials & Methods Measurements Instruments Protocols Software Developed Parts Submitted Characterization The Team Notebook Week-book Freezer Management Biological Safety Pavia History University Gallery Sponsors Gallery Team Locations		Protocols
	LB medium DNA resuspension from iGEM 2009 plates Transformation X-Gal staining protocol for beta galactosidase (blue/white screening) Glycerol stocks Plasmid extraction BioBrick digestion with restriction enzymes Electrophoresis DNA gel extraction DNA precipitation with sodium acetate Ligation PCR Sensing ethanol concentration: our do-it-yourself kit

DNA gel extraction

(Estimated time: 1 hour and 30 min)

Follow Roche standard protocol: Roche Agarose Gel DNA Extraction Kit protocol

Agarose gel with well-separated, sharp bands Scalpel and tweezers to cut gel (they have to be cleaned in ddH2O after every cut) Roche Agarose Gel DNA Extraction Kit

• Recent changes
• What links here
• Related changes
• Special pages
• My preferences

• Printable version
• Permanent link
• Privacy policy
• Disclaimers