Template:Team:KULeuven/7 September 2009/VanillinProduction

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  • Miniprep of the four colonies from EF ligation
    • Accidentally used double volumes of elution buffer on EF4
part concentration 260/280 260/230
EF 1 115,5 1,98 2,31
EF 2 105,2 1,96 1,66
EF 3 95,1 2,05 0,98
EF 4 52,7 2,06 0,97
  • Gel electrophoresis from restriction on sam5 and sam8 looked good
  • Purified and ligated the restriction from sam5 and sam8 overnight
    • Concentration of sam8 was barely enough for ligation...
part concentration 260/280 260/230
sam5 10,1 2,11 0,04
sam8 3,1 3,96 0,02
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